CE-LIF-MSn profiling of oligosaccharides in human milk and feces of breast-fed babies

S.A. Albrecht, H.A. Schols, E.G.H.M. van den Heuvel, A.G.J. Voragen, H. Gruppen

Research output: Contribution to journalArticleAcademicpeer-review

83 Citations (Scopus)

Abstract

Mixtures of the complex human milk oligosaccharides (HMOs) are difficult to analyze and gastrointestinal bioconversion products of HMOs may complicate analysis even more. Their analysis, therefore, requires the combination of a sensitive and high-resolution separation technique with a mass identification tool. This study introduces for the first time the hyphenation of CE with an electrospray mass spectrometer, capable to perform multiple MS analysis (ESI-MSn) for the separation and characterization of HMOs in breast milk and feces of breast-fed babies. LIF was used for on- and off-line detections. From the overall 47 peaks detected in off-line CE-LIF electropherograms, 21 peaks could be unambiguously and 11 peaks could be tentatively assigned. The detailed structural characterization of a novel lacto-N-neo-tetraose isomer and a novel lacto-N-fucopentaose isomer was established in baby feces and pointed to gastrointestinal hydrolysis of higher-Mw HMOs. CE-LIF-ESI-MSn presents, therefore, a useful tool which contributes to an advanced understanding on the fate of individual HMOs during their gastrointestinal passage.
Original languageEnglish
Pages (from-to)1264-1273
JournalElectrophoresis
Volume31
Issue number7
DOIs
Publication statusPublished - 2010

Keywords

  • induced fluorescence detection
  • capillary-electrophoresis
  • mass-spectrometry
  • fed infants
  • sialylated oligosaccharides
  • liquid-chromatography
  • preterm
  • lactose
  • electrochromatography
  • combination

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