Abstract
Verticillium spp. are soil-borne plant pathogens that are responsible for devastating wilt diseases in over 200 dicotyledonous plant species, including many economically important crops. Despite the economical importance of
Verticillium spp., little is known about the molecular basis of Verticillium pathogenicity and host resistance against this fungus. To identify crucial components involved in compatible and incompatible interactions between Verticillium and tomato, a comparative transcript profiling was performed on
susceptible (MM-S) and resistant (MM-R) tomato lines of the cultivar MoneyMaker. The MM-S plants were inoculated with a race-1 (MM-S1) or a race-2 (MM-S2) isolate of Verticillium, while MM-R plants were inoculated with a race-1 (MM-R1) isolate only. The complete cDNA-AFLP analysis of the
incompatible and compatible interaction was performed using 176 selective primer combinations. This allowed the visualization of 11,500 transcript-derived fragments (TDFs). Of these, 1434 TDFs (12.5%) showed differential regulation.
Two classes of differentially expressed TDFs (DE-TDFs) were defined; I) TDFs up-regulated in the incompatible interaction (302 TDFs) and II) TDFs down-regulated in the compatible interaction (634 TDFs). The DE-TDFs being up-regulated specifically in resistant tomato cultivar (MM-R1) may be
involved in the host resistance response following Verticillium perception, and these TDFs will be functionally analyzed using virus-induced gene silencing (VIGS). This study will help to identify crucial components involved in Verticillium
pathogenicity and host plant resistance. This research is supported by the Dutch Technology Foundation STW, applied science division of NWO and the Technology Program of the Ministry of Economic Affairs.
Verticillium spp., little is known about the molecular basis of Verticillium pathogenicity and host resistance against this fungus. To identify crucial components involved in compatible and incompatible interactions between Verticillium and tomato, a comparative transcript profiling was performed on
susceptible (MM-S) and resistant (MM-R) tomato lines of the cultivar MoneyMaker. The MM-S plants were inoculated with a race-1 (MM-S1) or a race-2 (MM-S2) isolate of Verticillium, while MM-R plants were inoculated with a race-1 (MM-R1) isolate only. The complete cDNA-AFLP analysis of the
incompatible and compatible interaction was performed using 176 selective primer combinations. This allowed the visualization of 11,500 transcript-derived fragments (TDFs). Of these, 1434 TDFs (12.5%) showed differential regulation.
Two classes of differentially expressed TDFs (DE-TDFs) were defined; I) TDFs up-regulated in the incompatible interaction (302 TDFs) and II) TDFs down-regulated in the compatible interaction (634 TDFs). The DE-TDFs being up-regulated specifically in resistant tomato cultivar (MM-R1) may be
involved in the host resistance response following Verticillium perception, and these TDFs will be functionally analyzed using virus-induced gene silencing (VIGS). This study will help to identify crucial components involved in Verticillium
pathogenicity and host plant resistance. This research is supported by the Dutch Technology Foundation STW, applied science division of NWO and the Technology Program of the Ministry of Economic Affairs.
| Original language | English |
|---|---|
| Title of host publication | ISMPMI International Congress abstracts, Quebec City, Canada, 19-23 July 2009 |
| Publisher | International Society for Molecular Plant-Microbe Interactions |
| Publication status | Published - 2009 |
| Event | XIV International Congress on Molecular Plant-Microbe Interactions, Quebec City, Canada - Duration: 19 Jul 2009 → 23 Jul 2009 |
Conference
| Conference | XIV International Congress on Molecular Plant-Microbe Interactions, Quebec City, Canada |
|---|---|
| Period | 19/07/09 → 23/07/09 |