Carrot arabinogalactan proteins are interlinked with pectins.

P. Immerzeel; M.M. Eppink; S.C. de Vries; H.A. Schols; A.G.J. Voragen

doi:10.1111/j.1399-3054.2006.00712.x

Carrot arabinogalactan proteins are interlinked with pectins.

P. Immerzeel, M.M. Eppink, S.C. de Vries, H.A. Schols, A.G.J. Voragen

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Cell wall extracts from a carrot cell culture and tap roots were obtained by sequential extraction with water, EDTA buffer solution and cold sodium hydroxide solution. Arabinogalactan proteins (AGPs) were isolated from the extracts and from the medium of the cell culture and analysed for their molecular weight distribution and carbohydrate composition. Copper ions were used to separate the Yariv positive fractions into AGP fractions with a high and a low level of galacturonic acid (GalA). The GalA rich AGP fractions were incubated with pectin methylesterase and polygalacturonase. This enzyme incubation released GalA fragments from the AGP fractions as monitored by HPAEC and MALDI-TOF MS. At least part of carrot AGPs from the medium and cell walls may be covalently linked to pectin containing a homogalacturonan structural element.

Original language	English
Pages (from-to)	18-28
Journal	Physiologia Plantarum
Volume	128
Issue number	1
DOIs	https://doi.org/10.1111/j.1399-3054.2006.00712.x
Publication status	Published - 2006

Keywords

cell-wall polysaccharides
daucus-carota
hairy regions
arabidopsis
suspension
plants
substances
culture
family
pulp

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10.1111/j.1399-3054.2006.00712.x

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title = "Carrot arabinogalactan proteins are interlinked with pectins.",

abstract = "Cell wall extracts from a carrot cell culture and tap roots were obtained by sequential extraction with water, EDTA buffer solution and cold sodium hydroxide solution. Arabinogalactan proteins (AGPs) were isolated from the extracts and from the medium of the cell culture and analysed for their molecular weight distribution and carbohydrate composition. Copper ions were used to separate the Yariv positive fractions into AGP fractions with a high and a low level of galacturonic acid (GalA). The GalA rich AGP fractions were incubated with pectin methylesterase and polygalacturonase. This enzyme incubation released GalA fragments from the AGP fractions as monitored by HPAEC and MALDI-TOF MS. At least part of carrot AGPs from the medium and cell walls may be covalently linked to pectin containing a homogalacturonan structural element.",

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author = "P. Immerzeel and M.M. Eppink and {de Vries}, S.C. and H.A. Schols and A.G.J. Voragen",

year = "2006",

doi = "10.1111/j.1399-3054.2006.00712.x",

language = "English",

volume = "128",

pages = "18--28",

journal = "Physiologia Plantarum",

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TY - JOUR

T1 - Carrot arabinogalactan proteins are interlinked with pectins.

AU - Immerzeel, P.

AU - Eppink, M.M.

AU - de Vries, S.C.

AU - Schols, H.A.

AU - Voragen, A.G.J.

PY - 2006

Y1 - 2006

N2 - Cell wall extracts from a carrot cell culture and tap roots were obtained by sequential extraction with water, EDTA buffer solution and cold sodium hydroxide solution. Arabinogalactan proteins (AGPs) were isolated from the extracts and from the medium of the cell culture and analysed for their molecular weight distribution and carbohydrate composition. Copper ions were used to separate the Yariv positive fractions into AGP fractions with a high and a low level of galacturonic acid (GalA). The GalA rich AGP fractions were incubated with pectin methylesterase and polygalacturonase. This enzyme incubation released GalA fragments from the AGP fractions as monitored by HPAEC and MALDI-TOF MS. At least part of carrot AGPs from the medium and cell walls may be covalently linked to pectin containing a homogalacturonan structural element.

AB - Cell wall extracts from a carrot cell culture and tap roots were obtained by sequential extraction with water, EDTA buffer solution and cold sodium hydroxide solution. Arabinogalactan proteins (AGPs) were isolated from the extracts and from the medium of the cell culture and analysed for their molecular weight distribution and carbohydrate composition. Copper ions were used to separate the Yariv positive fractions into AGP fractions with a high and a low level of galacturonic acid (GalA). The GalA rich AGP fractions were incubated with pectin methylesterase and polygalacturonase. This enzyme incubation released GalA fragments from the AGP fractions as monitored by HPAEC and MALDI-TOF MS. At least part of carrot AGPs from the medium and cell walls may be covalently linked to pectin containing a homogalacturonan structural element.

KW - cell-wall polysaccharides

KW - daucus-carota

KW - hairy regions

KW - arabidopsis

KW - suspension

KW - plants

KW - substances

KW - culture

KW - family

KW - pulp

U2 - 10.1111/j.1399-3054.2006.00712.x

DO - 10.1111/j.1399-3054.2006.00712.x

M3 - Article

SN - 0031-9317

VL - 128

SP - 18

EP - 28

JO - Physiologia Plantarum

JF - Physiologia Plantarum

IS - 1

ER -

Carrot arabinogalactan proteins are interlinked with pectins.

Abstract

Keywords

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