Carp macrophages and neutrophilic granulocytes secrete an interleukin-1-like factor.

B.M.L. Verburg-van Kemenade, F.A.A. Weyts, R. Debets, G. Flik

Research output: Contribution to journalArticleAcademicpeer-review

80 Citations (Scopus)

Abstract

Carp, Cyprinus carpio L, macrophages and neutrophilic granulocytes obtained from pronephros were cultured. Supernatant was harvested after 48 h and tested for interleukin-1 (IL-1) bioactivity. A concentration-dependent stimulation of proliferation was found of carp Ig− lymphocytes as well as of the murine IL-1 dependent D10 (N4) M cell line. A 4 h treatment of cells with phorbol myristate acetate prior to culture gave a two- to fourfold enhancement of the bioactivity in the supernatant. Antibodies raised in sheep against human recombinant IL-1α or IL-1β added to the supernatant annulled the Il-1 bioactivity. Western blot analysis of supernatants with sheep or rabbit polyclonal antisera against human Il-1s revealed 22 kDa and 15 kDa protein species. The predominant newly synthesized protein that was immunoprecipitated with these antisera was a 15 kDa molecular species. We conclude that carp macrophages and neutrophilic granulocytes produce an IL-1-like molecule with T-cell proliferating potency that shares structural similarities with mammalian IL-1. This is the first evidence for the IL-1 signal protein in carp immunocompetent cells.
Original languageEnglish
Pages (from-to)59-70
JournalDevelopmental and Comparative Immunology
Volume19
DOIs
Publication statusPublished - 1995

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