Carotenoid fluorescence in Dunaliella salina

D.M.M. Kleinegris, M.A. van Es, M.G.J. Janssen, W.A. Brandenburg, R.H. Wijffels

Research output: Contribution to journalArticleAcademicpeer-review

43 Citations (Scopus)

Abstract

Dunaliella salina is a halotolerant green alga that is well known for its carotenoid producing capacity. The produced carotenoids are mainly stored in lipid globules. For various research purposes, such as production and extraction kinetics, we would like to determine and/or localise the carotenoid globules in vivo. In this study, we show that the carotenoid-rich globules emit clear green fluorescence, which can be used in, for example, fluorescence microscopy (e.g. CLSM) to obtain pictures of the cells and their carotenoid content.
Original languageEnglish
Pages (from-to)645-649
JournalJournal of Applied Phycology
Volume22
Issue number5
DOIs
Publication statusPublished - 2010

Keywords

  • beta-carotene
  • spectroscopy
  • pigments

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