Carbohydrate Microarray on Glass: a Tool for Carbohydrate-Lectin Interactions

K.K.R. Tetala, M. Giesbers, G.M. Visser, E.J.R. Sudhölter, T.A. van Beek

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

A simple method to immobilize carbohydrates on a glass surface to obtain a carbohydrate microarray is described. The array was used to study carbohydrate-lectin interactions. The glass surface was modified with aldehyde terminated linker groups of various chain lengths. Coupling of carbohydrates with an amino terminated alkyl spacer to the aldehyde terminated glass followed by reductive amination resulted in carbohydrate microarrays. Fluorescently labeled (FI-TC) lectins (concanavalin A and Arachis hypogaea) were used to study specific carbohydrate-lectin interactions. contact angle, atomic force microscopy (AFM) and confocal laser fluorescence microscopy (CLFM) techniques were used in this study to monitor the modification of the glass and the successful selective binding of lectins to the carbohydrate microarray.
Original languageEnglish
Pages (from-to)391-394
JournalNatural Product Communications
Volume2
Issue number4
Publication statusPublished - 2007

Keywords

  • arrays
  • technology
  • proteins
  • binding

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