Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides

M.A. Kabel; W.H. Heijnis; E.J. Bakx; I.J. Kuijpers; A.G.J. Voragen; H.A. Schols

doi:10.1016/j.chroma.2006.10.058

Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Various plant polysaccharide derived mono- and oligosaccharides were derivatized with the fluorescent 9-aminopyrene-1,4,6-trisulfonate (APTS) and subjected to capillary electrophoresis (CE) in combination with laser induced fluorescence (LIF) detection. CE-LIF was suitable for mol-based quantification of various APTS-monosaccharides. CE-LIF of APTS-oligosaccharides showed high resolutions, while analysis times were at maximum 15 min. The coupling of CE to electrospray-iontrap mass spectrometery (MS) with online UV detection showed to be a powerful technique in the identification of APTS-oligosaccharides. For the first time, various APTS-xylo-oligosaccharides, having either no, O-acetyl, arabinosyl or xylosyl substitutions at varying positions, were identified by using CE-LIF and CE-MSn.

Original language	English
Pages (from-to)	119-126
Journal	Journal of Chromatography. A, Including electrophoresis and other separation methods
Volume	1137
Issue number	1
DOIs	https://doi.org/10.1016/j.chroma.2006.10.058
Publication status	Published - 2006

Keywords

laser-desorption/ionization-time
anion-exchange chromatography
treated eucalyptus wood
gel-electrophoresis
dietary fiber
wheat-flour
cell-walls
oligosaccharides
spectrometry
separation

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10.1016/j.chroma.2006.10.058

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Kabel, M. A., Heijnis, W. H., Bakx, E. J., Kuijpers, I. J., Voragen, A. G. J., & Schols, H. A. (2006). Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides. Journal of Chromatography. A, Including electrophoresis and other separation methods, 1137(1), 119-126. https://doi.org/10.1016/j.chroma.2006.10.058

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title = "Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides",

abstract = "Various plant polysaccharide derived mono- and oligosaccharides were derivatized with the fluorescent 9-aminopyrene-1,4,6-trisulfonate (APTS) and subjected to capillary electrophoresis (CE) in combination with laser induced fluorescence (LIF) detection. CE-LIF was suitable for mol-based quantification of various APTS-monosaccharides. CE-LIF of APTS-oligosaccharides showed high resolutions, while analysis times were at maximum 15 min. The coupling of CE to electrospray-iontrap mass spectrometery (MS) with online UV detection showed to be a powerful technique in the identification of APTS-oligosaccharides. For the first time, various APTS-xylo-oligosaccharides, having either no, O-acetyl, arabinosyl or xylosyl substitutions at varying positions, were identified by using CE-LIF and CE-MSn.",

keywords = "laser-desorption/ionization-time, anion-exchange chromatography, treated eucalyptus wood, gel-electrophoresis, dietary fiber, wheat-flour, cell-walls, oligosaccharides, spectrometry, separation",

author = "M.A. Kabel and W.H. Heijnis and E.J. Bakx and I.J. Kuijpers and A.G.J. Voragen and H.A. Schols",

year = "2006",

doi = "10.1016/j.chroma.2006.10.058",

language = "English",

volume = "1137",

pages = "119--126",

journal = "Journal of Chromatography. A, Including electrophoresis and other separation methods",

issn = "0021-9673",

publisher = "Elsevier",

number = "1",

}

Kabel, MA, Heijnis, WH, Bakx, EJ , Kuijpers, IJ , Voragen, AGJ & Schols, HA 2006, 'Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides', Journal of Chromatography. A, Including electrophoresis and other separation methods, vol. 1137, no. 1, pp. 119-126. https://doi.org/10.1016/j.chroma.2006.10.058

Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides. / Kabel, M.A.; Heijnis, W.H.; Bakx, E.J. et al.
In: Journal of Chromatography. A, Including electrophoresis and other separation methods, Vol. 1137, No. 1, 2006, p. 119-126.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides

AU - Kabel, M.A.

AU - Heijnis, W.H.

AU - Bakx, E.J.

AU - Kuijpers, I.J.

AU - Voragen, A.G.J.

AU - Schols, H.A.

PY - 2006

Y1 - 2006

N2 - Various plant polysaccharide derived mono- and oligosaccharides were derivatized with the fluorescent 9-aminopyrene-1,4,6-trisulfonate (APTS) and subjected to capillary electrophoresis (CE) in combination with laser induced fluorescence (LIF) detection. CE-LIF was suitable for mol-based quantification of various APTS-monosaccharides. CE-LIF of APTS-oligosaccharides showed high resolutions, while analysis times were at maximum 15 min. The coupling of CE to electrospray-iontrap mass spectrometery (MS) with online UV detection showed to be a powerful technique in the identification of APTS-oligosaccharides. For the first time, various APTS-xylo-oligosaccharides, having either no, O-acetyl, arabinosyl or xylosyl substitutions at varying positions, were identified by using CE-LIF and CE-MSn.

AB - Various plant polysaccharide derived mono- and oligosaccharides were derivatized with the fluorescent 9-aminopyrene-1,4,6-trisulfonate (APTS) and subjected to capillary electrophoresis (CE) in combination with laser induced fluorescence (LIF) detection. CE-LIF was suitable for mol-based quantification of various APTS-monosaccharides. CE-LIF of APTS-oligosaccharides showed high resolutions, while analysis times were at maximum 15 min. The coupling of CE to electrospray-iontrap mass spectrometery (MS) with online UV detection showed to be a powerful technique in the identification of APTS-oligosaccharides. For the first time, various APTS-xylo-oligosaccharides, having either no, O-acetyl, arabinosyl or xylosyl substitutions at varying positions, were identified by using CE-LIF and CE-MSn.

KW - laser-desorption/ionization-time

KW - anion-exchange chromatography

KW - treated eucalyptus wood

KW - gel-electrophoresis

KW - dietary fiber

KW - wheat-flour

KW - cell-walls

KW - oligosaccharides

KW - spectrometry

KW - separation

U2 - 10.1016/j.chroma.2006.10.058

DO - 10.1016/j.chroma.2006.10.058

M3 - Article

SN - 0021-9673

VL - 1137

SP - 119

EP - 126

JO - Journal of Chromatography. A, Including electrophoresis and other separation methods

JF - Journal of Chromatography. A, Including electrophoresis and other separation methods

IS - 1

ER -

Kabel MA, Heijnis WH, Bakx EJ , Kuijpers IJ , Voragen AGJ , Schols HA. Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides. Journal of Chromatography. A, Including electrophoresis and other separation methods. 2006;1137(1):119-126. doi: 10.1016/j.chroma.2006.10.058

Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides

Abstract

Keywords

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