Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides

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Abstract

Various plant polysaccharide derived mono- and oligosaccharides were derivatized with the fluorescent 9-aminopyrene-1,4,6-trisulfonate (APTS) and subjected to capillary electrophoresis (CE) in combination with laser induced fluorescence (LIF) detection. CE-LIF was suitable for mol-based quantification of various APTS-monosaccharides. CE-LIF of APTS-oligosaccharides showed high resolutions, while analysis times were at maximum 15 min. The coupling of CE to electrospray-iontrap mass spectrometery (MS) with online UV detection showed to be a powerful technique in the identification of APTS-oligosaccharides. For the first time, various APTS-xylo-oligosaccharides, having either no, O-acetyl, arabinosyl or xylosyl substitutions at varying positions, were identified by using CE-LIF and CE-MSn.
Original languageEnglish
Pages (from-to)119-126
JournalJournal of Chromatography. A, Including electrophoresis and other separation methods
Volume1137
Issue number1
DOIs
Publication statusPublished - 2006

Keywords

  • laser-desorption/ionization-time
  • anion-exchange chromatography
  • treated eucalyptus wood
  • gel-electrophoresis
  • dietary fiber
  • wheat-flour
  • cell-walls
  • oligosaccharides
  • spectrometry
  • separation

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