TY - JOUR
T1 - Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides
AU - Kabel, M.A.
AU - Heijnis, W.H.
AU - Bakx, E.J.
AU - Kuijpers, I.J.
AU - Voragen, A.G.J.
AU - Schols, H.A.
PY - 2006
Y1 - 2006
N2 - Various plant polysaccharide derived mono- and oligosaccharides were derivatized with the fluorescent 9-aminopyrene-1,4,6-trisulfonate (APTS) and subjected to capillary electrophoresis (CE) in combination with laser induced fluorescence (LIF) detection. CE-LIF was suitable for mol-based quantification of various APTS-monosaccharides. CE-LIF of APTS-oligosaccharides showed high resolutions, while analysis times were at maximum 15 min. The coupling of CE to electrospray-iontrap mass spectrometery (MS) with online UV detection showed to be a powerful technique in the identification of APTS-oligosaccharides. For the first time, various APTS-xylo-oligosaccharides, having either no, O-acetyl, arabinosyl or xylosyl substitutions at varying positions, were identified by using CE-LIF and CE-MSn.
AB - Various plant polysaccharide derived mono- and oligosaccharides were derivatized with the fluorescent 9-aminopyrene-1,4,6-trisulfonate (APTS) and subjected to capillary electrophoresis (CE) in combination with laser induced fluorescence (LIF) detection. CE-LIF was suitable for mol-based quantification of various APTS-monosaccharides. CE-LIF of APTS-oligosaccharides showed high resolutions, while analysis times were at maximum 15 min. The coupling of CE to electrospray-iontrap mass spectrometery (MS) with online UV detection showed to be a powerful technique in the identification of APTS-oligosaccharides. For the first time, various APTS-xylo-oligosaccharides, having either no, O-acetyl, arabinosyl or xylosyl substitutions at varying positions, were identified by using CE-LIF and CE-MSn.
KW - laser-desorption/ionization-time
KW - anion-exchange chromatography
KW - treated eucalyptus wood
KW - gel-electrophoresis
KW - dietary fiber
KW - wheat-flour
KW - cell-walls
KW - oligosaccharides
KW - spectrometry
KW - separation
U2 - 10.1016/j.chroma.2006.10.058
DO - 10.1016/j.chroma.2006.10.058
M3 - Article
SN - 0021-9673
VL - 1137
SP - 119
EP - 126
JO - Journal of Chromatography. A, Including electrophoresis and other separation methods
JF - Journal of Chromatography. A, Including electrophoresis and other separation methods
IS - 1
ER -