Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina)

Maarten J. Gilbert; Aldert L. Zomer; Arjen J. Timmerman; Mirlin P. Spaninks; Ana Rubio-García; John W. Rossen; Birgitta Duim; Jaap A. Wagenaar

doi:10.1099/ijsem.0.002742

Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina)

Maarten J. Gilbert^*, Aldert L. Zomer, Arjen J. Timmerman, Mirlin P. Spaninks, Ana Rubio-García, John W. Rossen, Birgitta Duim, Jaap A. Wagenaar

^*Corresponding author for this work

Epidemiology, Bio-informatics & Animal models

Research output: Contribution to journal › Article › Academic › peer-review

19 Citations (Scopus)

Abstract

During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobacter taxon were identified. Campylobacter isolates, which differed from the established Campylobacter taxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobacter taxa and most closely related to Campylobacter corcagiensis, Campylobacter geochelonis and Campylobacter ureolyticus. The isolates can be distinguished phenotypically from all other Campylobacter taxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter, for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5T (=LMG 30333T=CCUG 71276T).

Original language	English
Pages (from-to)	1787-1794
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	68
Issue number	5
DOIs	https://doi.org/10.1099/ijsem.0.002742
Publication status	Published - 1 May 2018

Keywords

Average nucleotide identity
Campylobacter
Common seal
Core genome phylogeny
Microbiome
Novel species

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title = "Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina)",

abstract = "During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobacter taxon were identified. Campylobacter isolates, which differed from the established Campylobacter taxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobacter taxa and most closely related to Campylobacter corcagiensis, Campylobacter geochelonis and Campylobacter ureolyticus. The isolates can be distinguished phenotypically from all other Campylobacter taxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter, for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5T (=LMG 30333T=CCUG 71276T).",

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author = "Gilbert, {Maarten J.} and Zomer, {Aldert L.} and Timmerman, {Arjen J.} and Spaninks, {Mirlin P.} and Ana Rubio-Garc{\'i}a and Rossen, {John W.} and Birgitta Duim and Wagenaar, {Jaap A.}",

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AU - Gilbert, Maarten J.

AU - Zomer, Aldert L.

AU - Timmerman, Arjen J.

AU - Spaninks, Mirlin P.

AU - Rubio-García, Ana

AU - Rossen, John W.

AU - Duim, Birgitta

AU - Wagenaar, Jaap A.

PY - 2018/5/1

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N2 - During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobacter taxon were identified. Campylobacter isolates, which differed from the established Campylobacter taxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobacter taxa and most closely related to Campylobacter corcagiensis, Campylobacter geochelonis and Campylobacter ureolyticus. The isolates can be distinguished phenotypically from all other Campylobacter taxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter, for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5T (=LMG 30333T=CCUG 71276T).

AB - During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobacter taxon were identified. Campylobacter isolates, which differed from the established Campylobacter taxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobacter taxa and most closely related to Campylobacter corcagiensis, Campylobacter geochelonis and Campylobacter ureolyticus. The isolates can be distinguished phenotypically from all other Campylobacter taxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter, for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5T (=LMG 30333T=CCUG 71276T).

KW - Average nucleotide identity

KW - Campylobacter

KW - Common seal

KW - Core genome phylogeny

KW - Microbiome

KW - Novel species

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DO - 10.1099/ijsem.0.002742

M3 - Article

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SN - 1466-5026

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JO - International Journal of Systematic and Evolutionary Microbiology

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IS - 5

ER -

Campylobacter blaseri sp. Nov., isolated from common seals (Phoca vitulina)

Abstract

Keywords

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