Caenorhabditis elegans males and hermaphrodites differ in susceptibility upon Orsay virus infection

L. van Sluijs, Jie Liu (Editor), Mels Schrama (Editor), Sanne van Hamond (Editor), G.P. Pijlman (Editor), J.E. Kammenga (Editor)

Research output: Contribution to conferencePosterAcademic

Abstract

Individuals differ in their susceptibility upon viral infection as a result of a complex interplay between the individuals genetic background and the environment. The genetic background determines the sex of an organism and affects a large range of traits. Here we show that the sex of Caenorhabdtis elegans affects the susceptibility upon infection with the intestinal pathogen the Orsay virus. We noticed that outbred, mixed male and hermaphrodite populations showed a lower viral load than inbred, hermaphrodite populations. This result suggested that either the method of breeding or the sex of the nematodes in the population affected the viral load. Therefore, males and hermaphrodites were separately infected. Populations of infected males showed a larger number of failed infections than hermaphrodites that originated from the same plate. Moreover, preliminary results suggest that the mechanism may be genotype-dependent. As Orsay virus infects nematodes after ingestion the difference in viral susceptibility may be determined by a different food intake by males and hermaphrodites. Yet, the food intake as measured by grinder movements is similar in males and hermaphrodites. Currently we are also quantifying the volume of food taken in by the male and hermaphrodite nematodes in liquid to get a better estimation of food intake during the viral infection. Besides, we are investigating if differences in molecular networks between both sexes can alter the viral susceptibility by measuring the transcriptional response upon infection. In nature C. elegans males may benefit from a higher resistance towards viruses and possibly other intestinal pathogens that are counteracted by similar mechanisms. An ecological advantage like increased pathogen resistance might be important for males in a species that suffers from outbreeding depression.
Original languageEnglish
Publication statusPublished - 5 Jul 2018
EventEcology, Evolution and Genomics of C. elegans and other nematodes - Wellcome Genome Campus, Hinxton, United Kingdom
Duration: 5 Jul 20187 Jul 2018

Conference

ConferenceEcology, Evolution and Genomics of C. elegans and other nematodes
CountryUnited Kingdom
CityHinxton
Period5/07/187/07/18

Fingerprint

Caenorhabditis elegans
Virus Diseases
Eating
Viruses
Viral Load
Population
Infection
Breeding
Genotype
Food

Cite this

van Sluijs, L., Liu, J. (Ed.), Schrama, M. (Ed.), van Hamond, S. (Ed.), Pijlman, G. P. (Ed.), & Kammenga, J. E. (Ed.) (2018). Caenorhabditis elegans males and hermaphrodites differ in susceptibility upon Orsay virus infection. Poster session presented at Ecology, Evolution and Genomics of C. elegans and other nematodes, Hinxton, United Kingdom.
van Sluijs, L. ; Liu, Jie (Editor) ; Schrama, Mels (Editor) ; van Hamond, Sanne (Editor) ; Pijlman, G.P. (Editor) ; Kammenga, J.E. (Editor). / Caenorhabditis elegans males and hermaphrodites differ in susceptibility upon Orsay virus infection. Poster session presented at Ecology, Evolution and Genomics of C. elegans and other nematodes, Hinxton, United Kingdom.
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van Sluijs, L, Liu, J (ed.), Schrama, M (ed.), van Hamond, S (ed.), Pijlman, GP (ed.) & Kammenga, JE (ed.) 2018, 'Caenorhabditis elegans males and hermaphrodites differ in susceptibility upon Orsay virus infection' Ecology, Evolution and Genomics of C. elegans and other nematodes, Hinxton, United Kingdom, 5/07/18 - 7/07/18, .

Caenorhabditis elegans males and hermaphrodites differ in susceptibility upon Orsay virus infection. / van Sluijs, L.; Liu, Jie (Editor); Schrama, Mels (Editor); van Hamond, Sanne (Editor); Pijlman, G.P. (Editor); Kammenga, J.E. (Editor).

2018. Poster session presented at Ecology, Evolution and Genomics of C. elegans and other nematodes, Hinxton, United Kingdom.

Research output: Contribution to conferencePosterAcademic

TY - CONF

T1 - Caenorhabditis elegans males and hermaphrodites differ in susceptibility upon Orsay virus infection

AU - van Sluijs, L.

A2 - Liu, Jie

A2 - Schrama, Mels

A2 - van Hamond, Sanne

A2 - Pijlman, G.P.

A2 - Kammenga, J.E.

PY - 2018/7/5

Y1 - 2018/7/5

N2 - Individuals differ in their susceptibility upon viral infection as a result of a complex interplay between the individuals genetic background and the environment. The genetic background determines the sex of an organism and affects a large range of traits. Here we show that the sex of Caenorhabdtis elegans affects the susceptibility upon infection with the intestinal pathogen the Orsay virus. We noticed that outbred, mixed male and hermaphrodite populations showed a lower viral load than inbred, hermaphrodite populations. This result suggested that either the method of breeding or the sex of the nematodes in the population affected the viral load. Therefore, males and hermaphrodites were separately infected. Populations of infected males showed a larger number of failed infections than hermaphrodites that originated from the same plate. Moreover, preliminary results suggest that the mechanism may be genotype-dependent. As Orsay virus infects nematodes after ingestion the difference in viral susceptibility may be determined by a different food intake by males and hermaphrodites. Yet, the food intake as measured by grinder movements is similar in males and hermaphrodites. Currently we are also quantifying the volume of food taken in by the male and hermaphrodite nematodes in liquid to get a better estimation of food intake during the viral infection. Besides, we are investigating if differences in molecular networks between both sexes can alter the viral susceptibility by measuring the transcriptional response upon infection. In nature C. elegans males may benefit from a higher resistance towards viruses and possibly other intestinal pathogens that are counteracted by similar mechanisms. An ecological advantage like increased pathogen resistance might be important for males in a species that suffers from outbreeding depression.

AB - Individuals differ in their susceptibility upon viral infection as a result of a complex interplay between the individuals genetic background and the environment. The genetic background determines the sex of an organism and affects a large range of traits. Here we show that the sex of Caenorhabdtis elegans affects the susceptibility upon infection with the intestinal pathogen the Orsay virus. We noticed that outbred, mixed male and hermaphrodite populations showed a lower viral load than inbred, hermaphrodite populations. This result suggested that either the method of breeding or the sex of the nematodes in the population affected the viral load. Therefore, males and hermaphrodites were separately infected. Populations of infected males showed a larger number of failed infections than hermaphrodites that originated from the same plate. Moreover, preliminary results suggest that the mechanism may be genotype-dependent. As Orsay virus infects nematodes after ingestion the difference in viral susceptibility may be determined by a different food intake by males and hermaphrodites. Yet, the food intake as measured by grinder movements is similar in males and hermaphrodites. Currently we are also quantifying the volume of food taken in by the male and hermaphrodite nematodes in liquid to get a better estimation of food intake during the viral infection. Besides, we are investigating if differences in molecular networks between both sexes can alter the viral susceptibility by measuring the transcriptional response upon infection. In nature C. elegans males may benefit from a higher resistance towards viruses and possibly other intestinal pathogens that are counteracted by similar mechanisms. An ecological advantage like increased pathogen resistance might be important for males in a species that suffers from outbreeding depression.

M3 - Poster

ER -

van Sluijs L, Liu J, (ed.), Schrama M, (ed.), van Hamond S, (ed.), Pijlman GP, (ed.), Kammenga JE, (ed.). Caenorhabditis elegans males and hermaphrodites differ in susceptibility upon Orsay virus infection. 2018. Poster session presented at Ecology, Evolution and Genomics of C. elegans and other nematodes, Hinxton, United Kingdom.