Cadmium toxicity in cultured tomato cells - Role of ethylene, proteases and oxidative stress in cell death signaling

E.T. Iakimova, E.J. Woltering, V.M. Kapchina-Toteva, F.J.M. Harren, S.M. Cristescu

    Research output: Contribution to journalArticleAcademicpeer-review

    50 Citations (Scopus)

    Abstract

    Our aim was to investigate the ability of cadmium to induce programmed cell death in tomato suspension cells and to determine the involvement of proteolysis, oxidative stress and ethylene. Tomato suspension cells were exposed to treatments with CdSO4 and cell death was calculated after fluorescein diacetate staining of the living cells. Ethylene was measured in a flow-through system using a laser-driven photo acoustic detector; hydrogen peroxide was determined by chemiluminescence in a ferricyanide-catalysed oxidation of luminol. We have demonstrated that cadmium induces cell death in tomato suspension cells involving caspase-like proteases, indicating that programmed cell death took place. Using range of inhibitors, we found that cysteine and serine peptidases, oxidative stress, calcium and ethylene are players in the cadmium-induced cell death signaling. Cadmium-induced cell death in tomato suspension cells exhibits morphological and biochemical similarities to plant hypersensitive response and to cadmium effects in animal systems.
    Original languageEnglish
    Pages (from-to)1521-1529
    JournalCell Biology International
    Volume32
    Issue number12
    DOIs
    Publication statusPublished - 2008

    Keywords

    • disease resistance response
    • caspase-like activity
    • hydrogen-peroxide
    • phytochelatin accumulation
    • phosphatidic-acid
    • suspension cells
    • tobacco cells
    • nitric-oxide
    • apoptosis
    • plants

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