Building an Artificial Stem Cell Niche: Prerequisites for Future 3D-Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology

Simon C. de Groot, Karen Sliedregt, Peter Paul G. van Benthem, Marcelo N. Rivolta, Margriet A. Huisman

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In recent years, there has been an increased interest in stem cells for the purpose of regenerative medicine to deliver a wide range of therapies to treat many diseases. However, two-dimensional cultures of stem cells are of limited use when studying the mechanism of pathogenesis of diseases and the feasibility of a treatment. Therefore, research is focusing on the strengths of stem cells in the three-dimensional (3D) structures mimicking organs, that is, organoids, or organ-on-chip, for modeling human biology and disease. As 3D technology advances, it is necessary to know which signals stem cells need to multiply and differentiate into complex structures. This holds especially true for the complex 3D structure of the inner ear. Recent work suggests that although other factors play a role, the extracellular matrix (ECM), including its topography, is crucial to mimic a stem cell niche in vitro and to drive stem cells toward the formation of the tissue of interest. Technological developments have led to the investigation of biomaterials that closely resemble the native ECM. In the fast forward moving research of organoids and organs-on-chip, the inner ear has hardly received attention. This review aims to provide an overview, by describing the general context in which cells, matrix and morphogens cooperate in order to build a tissue, to facilitate research in 3D inner ear technology. Anat Rec, 2019.

Original languageEnglish
Article numberar.24067
JournalThe Anatomical Record
DOIs
Publication statusE-pub ahead of print - 11 Jan 2019

Fingerprint

Artificial Cells
Stem Cell Niche
Inner Ear
biotechnology
Biotechnology
stem cells
ears
niche
niches
Stem Cells
stem
Organoids
matrix
Extracellular Matrix
extracellular matrix
Research
Technology
Regenerative Medicine
technological development
Biocompatible Materials

Keywords

  • 3D
  • biotechnology
  • inner ear
  • stem cell

Cite this

de Groot, Simon C. ; Sliedregt, Karen ; van Benthem, Peter Paul G. ; Rivolta, Marcelo N. ; Huisman, Margriet A. / Building an Artificial Stem Cell Niche: Prerequisites for Future 3D-Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology. In: The Anatomical Record. 2019.
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Building an Artificial Stem Cell Niche: Prerequisites for Future 3D-Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology. / de Groot, Simon C.; Sliedregt, Karen; van Benthem, Peter Paul G.; Rivolta, Marcelo N.; Huisman, Margriet A.

In: The Anatomical Record, 11.01.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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