Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands

Michiel V. Kroese, Lisa Beckers, Yvette J.W.M. Bisselink, Sophie Brasseur, Peter W. van Tulden, Miriam G.J. Koene, Hendrik I.J. Roest, Robin C. Ruuls, Jantien A. Backer, Jooske Ijzer, Joke W.B. van der Giessen, Peter T.J. Willemsen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. The overall prevalence of Brucella spp. antibodies in sera from healthy wild grey seals (Halichoerus grypus; n=11) and harbor seals (Phoca vitulina; n=40), collected between 2007 and 2013 ranged from 25% to 43%. Additionally, tissue samples of harbor seals collected along the Dutch shores between 2009 and 2012, were tested for the presence of Brucella spp. In total, 77% (30/ 39) seals were found to be positive for Brucella by IS711 real-time PCR in one or more tissue samples, including pulmonary nematodes. Viable Brucella was cultured from 40% (12/30) real-time PCR-positive seals, and was isolated from liver, lung, pulmonary lymph node, pulmonary nematode, or spleen, but not from any PCR-negative seals. Tissue samples from lung and pulmonary lymph nodes were the main source of viable Brucella bacteria. All isolates were typed as B. pinnipedialis by multiple-locus variable number of tandem repeats analysis-16 clustering and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and of sequence type ST25 by multilocus sequence typing analysis. No correlation was observed between Brucella infection and pathology. This report displays the isolation and identification of B. pinnipedialis in marine mammals in the Dutch part of the Atlantic Ocean.

Original languageEnglish
Pages (from-to)439-449
JournalJournal of Wildlife Diseases
Volume54
Issue number3
DOIs
Publication statusPublished - 1 Jul 2018

Fingerprint

Halichoerus grypus
Phoca vitulina
Brucella
seals
Netherlands
harbor
marine mammal
pathology
lungs
nematode
brucellosis
animal
marine mammals
antibody
serum
livestock
desorption
ionization
mass spectrometry
lymph nodes

Keywords

  • Brucella pinnipedialis
  • Halichoerus grypus
  • MALDI-TOF MS
  • Marine mammals
  • MLST
  • MLVA-16
  • Phoca vitulina
  • The Netherlands

Cite this

@article{29bf13abdf69462b827d3463e58aeeab,
title = "Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands",
abstract = "Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. The overall prevalence of Brucella spp. antibodies in sera from healthy wild grey seals (Halichoerus grypus; n=11) and harbor seals (Phoca vitulina; n=40), collected between 2007 and 2013 ranged from 25{\%} to 43{\%}. Additionally, tissue samples of harbor seals collected along the Dutch shores between 2009 and 2012, were tested for the presence of Brucella spp. In total, 77{\%} (30/ 39) seals were found to be positive for Brucella by IS711 real-time PCR in one or more tissue samples, including pulmonary nematodes. Viable Brucella was cultured from 40{\%} (12/30) real-time PCR-positive seals, and was isolated from liver, lung, pulmonary lymph node, pulmonary nematode, or spleen, but not from any PCR-negative seals. Tissue samples from lung and pulmonary lymph nodes were the main source of viable Brucella bacteria. All isolates were typed as B. pinnipedialis by multiple-locus variable number of tandem repeats analysis-16 clustering and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and of sequence type ST25 by multilocus sequence typing analysis. No correlation was observed between Brucella infection and pathology. This report displays the isolation and identification of B. pinnipedialis in marine mammals in the Dutch part of the Atlantic Ocean.",
keywords = "Brucella pinnipedialis, Halichoerus grypus, MALDI-TOF MS, Marine mammals, MLST, MLVA-16, Phoca vitulina, The Netherlands",
author = "Kroese, {Michiel V.} and Lisa Beckers and Bisselink, {Yvette J.W.M.} and Sophie Brasseur and {van Tulden}, {Peter W.} and Koene, {Miriam G.J.} and Roest, {Hendrik I.J.} and Ruuls, {Robin C.} and Backer, {Jantien A.} and Jooske Ijzer and {van der Giessen}, {Joke W.B.} and Willemsen, {Peter T.J.}",
year = "2018",
month = "7",
day = "1",
doi = "10.7589/2017-05-097",
language = "English",
volume = "54",
pages = "439--449",
journal = "Journal of Wildlife Diseases",
issn = "0090-3558",
number = "3",

}

Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands. / Kroese, Michiel V.; Beckers, Lisa; Bisselink, Yvette J.W.M.; Brasseur, Sophie; van Tulden, Peter W.; Koene, Miriam G.J.; Roest, Hendrik I.J.; Ruuls, Robin C.; Backer, Jantien A.; Ijzer, Jooske; van der Giessen, Joke W.B.; Willemsen, Peter T.J.

In: Journal of Wildlife Diseases, Vol. 54, No. 3, 01.07.2018, p. 439-449.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands

AU - Kroese, Michiel V.

AU - Beckers, Lisa

AU - Bisselink, Yvette J.W.M.

AU - Brasseur, Sophie

AU - van Tulden, Peter W.

AU - Koene, Miriam G.J.

AU - Roest, Hendrik I.J.

AU - Ruuls, Robin C.

AU - Backer, Jantien A.

AU - Ijzer, Jooske

AU - van der Giessen, Joke W.B.

AU - Willemsen, Peter T.J.

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. The overall prevalence of Brucella spp. antibodies in sera from healthy wild grey seals (Halichoerus grypus; n=11) and harbor seals (Phoca vitulina; n=40), collected between 2007 and 2013 ranged from 25% to 43%. Additionally, tissue samples of harbor seals collected along the Dutch shores between 2009 and 2012, were tested for the presence of Brucella spp. In total, 77% (30/ 39) seals were found to be positive for Brucella by IS711 real-time PCR in one or more tissue samples, including pulmonary nematodes. Viable Brucella was cultured from 40% (12/30) real-time PCR-positive seals, and was isolated from liver, lung, pulmonary lymph node, pulmonary nematode, or spleen, but not from any PCR-negative seals. Tissue samples from lung and pulmonary lymph nodes were the main source of viable Brucella bacteria. All isolates were typed as B. pinnipedialis by multiple-locus variable number of tandem repeats analysis-16 clustering and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and of sequence type ST25 by multilocus sequence typing analysis. No correlation was observed between Brucella infection and pathology. This report displays the isolation and identification of B. pinnipedialis in marine mammals in the Dutch part of the Atlantic Ocean.

AB - Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. The overall prevalence of Brucella spp. antibodies in sera from healthy wild grey seals (Halichoerus grypus; n=11) and harbor seals (Phoca vitulina; n=40), collected between 2007 and 2013 ranged from 25% to 43%. Additionally, tissue samples of harbor seals collected along the Dutch shores between 2009 and 2012, were tested for the presence of Brucella spp. In total, 77% (30/ 39) seals were found to be positive for Brucella by IS711 real-time PCR in one or more tissue samples, including pulmonary nematodes. Viable Brucella was cultured from 40% (12/30) real-time PCR-positive seals, and was isolated from liver, lung, pulmonary lymph node, pulmonary nematode, or spleen, but not from any PCR-negative seals. Tissue samples from lung and pulmonary lymph nodes were the main source of viable Brucella bacteria. All isolates were typed as B. pinnipedialis by multiple-locus variable number of tandem repeats analysis-16 clustering and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and of sequence type ST25 by multilocus sequence typing analysis. No correlation was observed between Brucella infection and pathology. This report displays the isolation and identification of B. pinnipedialis in marine mammals in the Dutch part of the Atlantic Ocean.

KW - Brucella pinnipedialis

KW - Halichoerus grypus

KW - MALDI-TOF MS

KW - Marine mammals

KW - MLST

KW - MLVA-16

KW - Phoca vitulina

KW - The Netherlands

U2 - 10.7589/2017-05-097

DO - 10.7589/2017-05-097

M3 - Article

VL - 54

SP - 439

EP - 449

JO - Journal of Wildlife Diseases

JF - Journal of Wildlife Diseases

SN - 0090-3558

IS - 3

ER -