A Gram-positive-staining, rod-shaped, facultatively oligotrophic bacterial strain, designated MB18(T), was isolated from a water sample collected from the River Mahananda at Siliguri (26 degrees 44' 23.20' N, 88 degrees 25' 22.89' a West-Bengal, India. On the basis of 16S rRNA gene sequence similarity, the closest relative of this strain was Brevibacterium epidermidis NCDO 2286(T) (96% similarity). The DNA G+C content of strain MB18(T) was 64.6 molo/o. Chemotaxonomic data [MK-8(H-2) as the major menaquinone, galactose as the sole cell-wall sugar, meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, phosphatidylglycerol and diphosphatidylglycerol as constituents of the polar lipids, anteiso-C-15:0, anteiso-C-17:0 and iso-C-15:0 as the major fatty acids] supported the affiliation of strain MB18T to the genus Brevibacterium. The results of DNA G +C content, 16S rRNA gene sequence analysis and biochemical and physiological analyses allowed genotypic and phenotypic differentiation of strain MB18(T) from its nearest neighbour B. epidermidis. The isolate therefore represents a novel species, for which the name Brevibacterium siliguriense sp. nov. is proposed; the type strain is MB18(T) (=DSM 23676(T)=LMG 25772(T)).
|Journal||International Journal of Systematic and Evolutionary Microbiology|
|Issue number||Pt 2|
|Publication status||Published - 2013|
- rapid method
Kumar, A., Ince, I. A., Kati, A., & Chakraborty, R. (2013). Brevibacterium siliguriense sp nov., a facultatively oligotrophic bacterium isolated from river water. International Journal of Systematic and Evolutionary Microbiology, 63(Pt 2), 511-515. https://doi.org/10.1099/ijs.0.038281-0