Breeding for pepper fruit quality: a genitical metabolomics approach

Y. Wahyuni

Research output: Thesisinternal PhD, WU

Abstract

 

A diverse collection of 32 pepper accessions was analysed for variation in health-related metabolites, such as carotenoids, capsaicinoids, flavonoids and vitamins C and E. For each of the metabolites analysed, there was a lot of variation among the accessions and it was possible to identify accessions with high amounts of specific metabolites. While all accessions contained high levels of vitamin C, accession C. chinenseAC2212 was found to be an excellent source of vitamin E, whereas C. annuumLong Sweet accumulated high levels of the flavonoid quercetin. A genetical metabolomics approach was used to study the genetic basis of metabolic traits in a segregating F2 population based on a cross between two contrasting pepper genotypes. This led to the identification of several metabolites QTL hotspots. The genetic basis for the accumulation of several flavonoids in pepper fruit was further investigated, by combining metabolic, gene expression and candidate gene-based marker data. This provided valuable insight into the key genes important for flavonoid accumulation in pepper fruit. The results of this study will help breeders to assist future breeding programs aimed at optimizing the levels of nutritional compounds in pepper fruit.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Bino, Raoul, Promotor
  • Bovy, Arnaud, Co-promotor
  • Sudarmonowati, E., Co-promotor, External person
  • Ballester, A.R., Co-promotor, External person
Award date4 Jun 2014
Place of PublicationWageningen
Publisher
Print ISBNs9789461739582
Publication statusPublished - 2014

Fingerprint

metabolomics
pepper
fruit quality
flavonoids
breeding
metabolites
fruits
vitamin E
ascorbic acid
quercetin
quantitative trait loci
carotenoids
genes
gene expression
genotype

Keywords

  • capsicum annuum
  • capsicum frutescens
  • capsicum chinense
  • capsicum baccatum
  • plant breeding
  • metabolomics
  • crop quality
  • secondary metabolites
  • health
  • genetic mapping
  • ripening

Cite this

Wahyuni, Y. (2014). Breeding for pepper fruit quality: a genitical metabolomics approach. Wageningen: Wageningen University.
Wahyuni, Y.. / Breeding for pepper fruit quality: a genitical metabolomics approach. Wageningen : Wageningen University, 2014. 193 p.
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title = "Breeding for pepper fruit quality: a genitical metabolomics approach",
abstract = "  A diverse collection of 32 pepper accessions was analysed for variation in health-related metabolites, such as carotenoids, capsaicinoids, flavonoids and vitamins C and E. For each of the metabolites analysed, there was a lot of variation among the accessions and it was possible to identify accessions with high amounts of specific metabolites. While all accessions contained high levels of vitamin C, accession C. chinenseAC2212 was found to be an excellent source of vitamin E, whereas C. annuumLong Sweet accumulated high levels of the flavonoid quercetin. A genetical metabolomics approach was used to study the genetic basis of metabolic traits in a segregating F2 population based on a cross between two contrasting pepper genotypes. This led to the identification of several metabolites QTL hotspots. The genetic basis for the accumulation of several flavonoids in pepper fruit was further investigated, by combining metabolic, gene expression and candidate gene-based marker data. This provided valuable insight into the key genes important for flavonoid accumulation in pepper fruit. The results of this study will help breeders to assist future breeding programs aimed at optimizing the levels of nutritional compounds in pepper fruit.",
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Wahyuni, Y 2014, 'Breeding for pepper fruit quality: a genitical metabolomics approach', Doctor of Philosophy, Wageningen University, Wageningen.

Breeding for pepper fruit quality: a genitical metabolomics approach. / Wahyuni, Y.

Wageningen : Wageningen University, 2014. 193 p.

Research output: Thesisinternal PhD, WU

TY - THES

T1 - Breeding for pepper fruit quality: a genitical metabolomics approach

AU - Wahyuni, Y.

N1 - WU thesis 5767

PY - 2014

Y1 - 2014

N2 -   A diverse collection of 32 pepper accessions was analysed for variation in health-related metabolites, such as carotenoids, capsaicinoids, flavonoids and vitamins C and E. For each of the metabolites analysed, there was a lot of variation among the accessions and it was possible to identify accessions with high amounts of specific metabolites. While all accessions contained high levels of vitamin C, accession C. chinenseAC2212 was found to be an excellent source of vitamin E, whereas C. annuumLong Sweet accumulated high levels of the flavonoid quercetin. A genetical metabolomics approach was used to study the genetic basis of metabolic traits in a segregating F2 population based on a cross between two contrasting pepper genotypes. This led to the identification of several metabolites QTL hotspots. The genetic basis for the accumulation of several flavonoids in pepper fruit was further investigated, by combining metabolic, gene expression and candidate gene-based marker data. This provided valuable insight into the key genes important for flavonoid accumulation in pepper fruit. The results of this study will help breeders to assist future breeding programs aimed at optimizing the levels of nutritional compounds in pepper fruit.

AB -   A diverse collection of 32 pepper accessions was analysed for variation in health-related metabolites, such as carotenoids, capsaicinoids, flavonoids and vitamins C and E. For each of the metabolites analysed, there was a lot of variation among the accessions and it was possible to identify accessions with high amounts of specific metabolites. While all accessions contained high levels of vitamin C, accession C. chinenseAC2212 was found to be an excellent source of vitamin E, whereas C. annuumLong Sweet accumulated high levels of the flavonoid quercetin. A genetical metabolomics approach was used to study the genetic basis of metabolic traits in a segregating F2 population based on a cross between two contrasting pepper genotypes. This led to the identification of several metabolites QTL hotspots. The genetic basis for the accumulation of several flavonoids in pepper fruit was further investigated, by combining metabolic, gene expression and candidate gene-based marker data. This provided valuable insight into the key genes important for flavonoid accumulation in pepper fruit. The results of this study will help breeders to assist future breeding programs aimed at optimizing the levels of nutritional compounds in pepper fruit.

KW - capsicum annuum

KW - capsicum frutescens

KW - capsicum chinense

KW - capsicum baccatum

KW - plantenveredeling

KW - metabolomica

KW - gewaskwaliteit

KW - secundaire metabolieten

KW - gezondheid

KW - genetische kartering

KW - rijp worden

KW - capsicum annuum

KW - capsicum frutescens

KW - capsicum chinense

KW - capsicum baccatum

KW - plant breeding

KW - metabolomics

KW - crop quality

KW - secondary metabolites

KW - health

KW - genetic mapping

KW - ripening

M3 - internal PhD, WU

SN - 9789461739582

PB - Wageningen University

CY - Wageningen

ER -

Wahyuni Y. Breeding for pepper fruit quality: a genitical metabolomics approach. Wageningen: Wageningen University, 2014. 193 p.