Abstract
A diverse collection of 32 pepper accessions was analysed for variation in health-related metabolites, such as carotenoids, capsaicinoids, flavonoids and vitamins C and E. For each of the metabolites analysed, there was a lot of variation among the accessions and it was possible to identify accessions with high amounts of specific metabolites. While all accessions contained high levels of vitamin C, accession C. chinenseAC2212 was found to be an excellent source of vitamin E, whereas C. annuumLong Sweet accumulated high levels of the flavonoid quercetin. A genetical metabolomics approach was used to study the genetic basis of metabolic traits in a segregating F2 population based on a cross between two contrasting pepper genotypes. This led to the identification of several metabolites QTL hotspots. The genetic basis for the accumulation of several flavonoids in pepper fruit was further investigated, by combining metabolic, gene expression and candidate gene-based marker data. This provided valuable insight into the key genes important for flavonoid accumulation in pepper fruit. The results of this study will help breeders to assist future breeding programs aimed at optimizing the levels of nutritional compounds in pepper fruit.
| Original language | English |
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| Qualification | Doctor of Philosophy |
| Awarding Institution |
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| Supervisors/Advisors |
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| Award date | 4 Jun 2014 |
| Place of Publication | Wageningen |
| Publisher | |
| Print ISBNs | 9789461739582 |
| Publication status | Published - 2014 |
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Keywords
- capsicum annuum
- capsicum frutescens
- capsicum chinense
- capsicum baccatum
- plant breeding
- metabolomics
- crop quality
- secondary metabolites
- health
- genetic mapping
- ripening
Cite this
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Breeding for pepper fruit quality: a genitical metabolomics approach. / Wahyuni, Y.
Wageningen : Wageningen University, 2014. 193 p.Research output: Thesis › internal PhD, WU
TY - THES
T1 - Breeding for pepper fruit quality: a genitical metabolomics approach
AU - Wahyuni, Y.
N1 - WU thesis 5767
PY - 2014
Y1 - 2014
N2 - A diverse collection of 32 pepper accessions was analysed for variation in health-related metabolites, such as carotenoids, capsaicinoids, flavonoids and vitamins C and E. For each of the metabolites analysed, there was a lot of variation among the accessions and it was possible to identify accessions with high amounts of specific metabolites. While all accessions contained high levels of vitamin C, accession C. chinenseAC2212 was found to be an excellent source of vitamin E, whereas C. annuumLong Sweet accumulated high levels of the flavonoid quercetin. A genetical metabolomics approach was used to study the genetic basis of metabolic traits in a segregating F2 population based on a cross between two contrasting pepper genotypes. This led to the identification of several metabolites QTL hotspots. The genetic basis for the accumulation of several flavonoids in pepper fruit was further investigated, by combining metabolic, gene expression and candidate gene-based marker data. This provided valuable insight into the key genes important for flavonoid accumulation in pepper fruit. The results of this study will help breeders to assist future breeding programs aimed at optimizing the levels of nutritional compounds in pepper fruit.
AB - A diverse collection of 32 pepper accessions was analysed for variation in health-related metabolites, such as carotenoids, capsaicinoids, flavonoids and vitamins C and E. For each of the metabolites analysed, there was a lot of variation among the accessions and it was possible to identify accessions with high amounts of specific metabolites. While all accessions contained high levels of vitamin C, accession C. chinenseAC2212 was found to be an excellent source of vitamin E, whereas C. annuumLong Sweet accumulated high levels of the flavonoid quercetin. A genetical metabolomics approach was used to study the genetic basis of metabolic traits in a segregating F2 population based on a cross between two contrasting pepper genotypes. This led to the identification of several metabolites QTL hotspots. The genetic basis for the accumulation of several flavonoids in pepper fruit was further investigated, by combining metabolic, gene expression and candidate gene-based marker data. This provided valuable insight into the key genes important for flavonoid accumulation in pepper fruit. The results of this study will help breeders to assist future breeding programs aimed at optimizing the levels of nutritional compounds in pepper fruit.
KW - capsicum annuum
KW - capsicum frutescens
KW - capsicum chinense
KW - capsicum baccatum
KW - plantenveredeling
KW - metabolomica
KW - gewaskwaliteit
KW - secundaire metabolieten
KW - gezondheid
KW - genetische kartering
KW - rijp worden
KW - capsicum annuum
KW - capsicum frutescens
KW - capsicum chinense
KW - capsicum baccatum
KW - plant breeding
KW - metabolomics
KW - crop quality
KW - secondary metabolites
KW - health
KW - genetic mapping
KW - ripening
M3 - internal PhD, WU
SN - 9789461739582
PB - Wageningen University
CY - Wageningen
ER -