Branched arabino-oligosaccharides isolated from sugar beet arabinan

Y. Westphal, S. Kuhnel, P. de Waard, S.W.A. Hinz, H.A. Schols, A.G.J. Voragen, H. Gruppen

Research output: Contribution to journalArticleAcademicpeer-review

72 Citations (Scopus)

Abstract

Sugar beet arabinan consists of an a-(1,5)-linked backbone of l-arabinosyl residues, which can be either single or double substituted with a-(1,2)- and/or a-(1,3)-linked l-arabinosyl residues. Neutral branched arabino-oligosaccharides were isolated from sugar beet arabinan by enzymatic degradation with mixtures of pure and well-defined arabinohydrolases from Chrysosporium lucknowense followed by fractionation based on size and analysis by MALDI-TOF MS and HPAEC. Using NMR analysis, two main series of branched arabino-oligosaccharides have been identified, both having an a-(1,5)-linked backbone of l-arabinosyl residues. One series carries single substituted a-(1,3)-linked l-arabinosyl residues at the backbone, whereas the other series consists of a double substituted a-(1,2,3,5)-linked arabinan structure within the molecule. The structures of eight such branched arabino-oligosaccharides were established.
Original languageEnglish
Pages (from-to)1180-1189
JournalCarbohydrate Research : an international journal
Volume345
Issue number9
DOIs
Publication statusPublished - 2010

Keywords

  • cell-walls
  • structural characterization
  • feruloyl oligosaccharides
  • polysaccharides
  • acid
  • nmr
  • pectins
  • pulp

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