Bracon brevicornis genome showcases the potential of linked-read sequencing in identifying a putative Complementary sex determiner gene

Kim B. Ferguson; Bart A. Pannebakker; Alejandra Centurión; Joost van den Heuvel; Ronald Nieuwenhuis; Frank F.M. Becker; Elio Schijlen; Andra Thiel; Bas J. Zwaan; Eveline C. Verhulst

doi:10.3390/genes11121390

Bracon brevicornis genome showcases the potential of linked-read sequencing in identifying a putative Complementary sex determiner gene

Kim B. Ferguson^*, Bart A. Pannebakker, Alejandra Centurión, Joost van den Heuvel, Ronald Nieuwenhuis, Frank F.M. Becker, Elio Schijlen, Andra Thiel, Bas J. Zwaan, Eveline C. Verhulst

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

Bracon brevicornis is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, Ostrinia nubilalis. It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read-based genome of B. brevicornis, complete with an ab initio-derived annotation and protein comparisons with fellow braconids, Fopius arisanus and Diachasma alloeum. We demonstrate the potential of linked-read assemblies in exploring regions of heterozygosity and search for structural and homology-derived evidence of the complementary sex determiner gene (csd).

Original language	English
Article number	1390
Pages (from-to)	1-13
Number of pages	13
Journal	Genes
Volume	11
Issue number	12
DOIs	https://doi.org/10.3390/genes11121390
Publication status	Published - Dec 2020

Keywords

10X Genomics
Braconinae
Complementary sex determination
Feminizer
Microsynteny
Parasitic wasp
Parasitoid

Access to Document

10.3390/genes11121390Licence: CC BY

The Bracon brevicornis genome, supporting data
Pannebakker, B. (Creator) & Ferguson, K. (Creator), Wageningen University & Research, 9 Dec 2019
DOI: 10.17026/dans-xn6-pjm8
Dataset
Linked-read genome of Bracon brevicornis, a parasitoid wasp, with annotation
Ferguson, K. (Creator), Pannebakker, B. (Creator), Centurión, A. (Creator), van den Heuvel, J. (Creator), Nieuwenhuis, R. (Creator), Becker, F. (Creator), Schijlen, E. (Creator), Thiel, A. (Creator), Zwaan, B. (Creator) & Verhulst, E. (Creator), Wageningen University, 6 Jan 2020
https://www.ebi.ac.uk/ena/browser/view/PRJEB35412
Dataset

BINGO: Breeding Invertebrates for Next Generation BioControl (BINGO)
1/01/15 → 31/12/18
Project: EU research project

Cite this

Ferguson, K. B., Pannebakker, B. A., Centurión, A., van den Heuvel, J., Nieuwenhuis, R., Becker, F. F. M., Schijlen, E., Thiel, A., Zwaan, B. J., & Verhulst, E. C. (2020). Bracon brevicornis genome showcases the potential of linked-read sequencing in identifying a putative Complementary sex determiner gene. Genes, 11(12), 1-13. Article 1390. https://doi.org/10.3390/genes11121390

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abstract = "Bracon brevicornis is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, Ostrinia nubilalis. It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read-based genome of B. brevicornis, complete with an ab initio-derived annotation and protein comparisons with fellow braconids, Fopius arisanus and Diachasma alloeum. We demonstrate the potential of linked-read assemblies in exploring regions of heterozygosity and search for structural and homology-derived evidence of the complementary sex determiner gene (csd).",

keywords = "10X Genomics, Braconinae, Complementary sex determination, Feminizer, Microsynteny, Parasitic wasp, Parasitoid",

author = "Ferguson, {Kim B.} and Pannebakker, {Bart A.} and Alejandra Centuri{\'o}n and {van den Heuvel}, Joost and Ronald Nieuwenhuis and Becker, {Frank F.M.} and Elio Schijlen and Andra Thiel and Zwaan, {Bas J.} and Verhulst, {Eveline C.}",

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doi = "10.3390/genes11121390",

language = "English",

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T1 - Bracon brevicornis genome showcases the potential of linked-read sequencing in identifying a putative Complementary sex determiner gene

AU - Ferguson, Kim B.

AU - Pannebakker, Bart A.

AU - Centurión, Alejandra

AU - van den Heuvel, Joost

AU - Nieuwenhuis, Ronald

AU - Becker, Frank F.M.

AU - Schijlen, Elio

AU - Thiel, Andra

AU - Zwaan, Bas J.

AU - Verhulst, Eveline C.

PY - 2020/12

Y1 - 2020/12

N2 - Bracon brevicornis is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, Ostrinia nubilalis. It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read-based genome of B. brevicornis, complete with an ab initio-derived annotation and protein comparisons with fellow braconids, Fopius arisanus and Diachasma alloeum. We demonstrate the potential of linked-read assemblies in exploring regions of heterozygosity and search for structural and homology-derived evidence of the complementary sex determiner gene (csd).

AB - Bracon brevicornis is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, Ostrinia nubilalis. It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read-based genome of B. brevicornis, complete with an ab initio-derived annotation and protein comparisons with fellow braconids, Fopius arisanus and Diachasma alloeum. We demonstrate the potential of linked-read assemblies in exploring regions of heterozygosity and search for structural and homology-derived evidence of the complementary sex determiner gene (csd).

KW - 10X Genomics

KW - Braconinae

KW - Complementary sex determination

KW - Feminizer

KW - Microsynteny

KW - Parasitic wasp

KW - Parasitoid

U2 - 10.3390/genes11121390

DO - 10.3390/genes11121390

M3 - Article

C2 - 33255162

AN - SCOPUS:85096653996

SN - 2073-4425

VL - 11

SP - 1

EP - 13

JO - Genes

JF - Genes

IS - 12

M1 - 1390

ER -

Bracon brevicornis genome showcases the potential of linked-read sequencing in identifying a putative Complementary sex determiner gene

Abstract

Keywords

Access to Document

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Datasets

The Bracon brevicornis genome, supporting data

Linked-read genome of Bracon brevicornis, a parasitoid wasp, with annotation

Projects

BINGO: Breeding Invertebrates for Next Generation BioControl (BINGO)

Cite this