Bovine natural killer cells are present in Escherichia coli infected mammary gland tissue and show antimicrobial activity in vitro

Anja Sipka, Brianna Pomeroy, Suzanne Klaessig, Ynte Schukken

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Natural killer (NK) cells are early responders in bacterial infections but their role in bovine mastitis has not been characterized. For the first time, we show the presence of NK cells (NKp46+/CD3) in bovine mammary gland tissue after an intramammary challenge with Escherichia (E.) coli. A small number of NK cells was detected in milk from quarters before and during an E. coli challenge. In vitro cultures of primary bovine mammary gland epithelial cells stimulated with UV irradiated E. coli induced significant migration of peripheral blood NK cells (pbNK) within 2 h. Furthermore, pbNK cells significantly reduced counts of live E. coli in vitro within 2 h of culture. The results show that bovine NK cells have the capacity to migrate to the site of infection and produce antibacterial mediators. These findings introduce NK cells as a leukocyte population in the mammary gland with potential functions in the innate immune response in bovine mastitis.

Original languageEnglish
Pages (from-to)54-60
JournalComparative Immunology Microbiology and Infectious Diseases
Volume48
DOIs
Publication statusPublished - 2016

Fingerprint

natural killer cells
Human Mammary Glands
mammary glands
Natural Killer Cells
anti-infective agents
Escherichia coli
cattle
Bovine Mastitis
bovine mastitis
blood cells
Blood Cells
Escherichia
tissues
In Vitro Techniques
bacterial infections
Bacterial Infections
Innate Immunity
in vitro culture
leukocytes
Milk

Keywords

  • Bovine mastitis
  • E. coli
  • Innate immunity
  • Mammary gland epithelial cells
  • NK cells

Cite this

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title = "Bovine natural killer cells are present in Escherichia coli infected mammary gland tissue and show antimicrobial activity in vitro",
abstract = "Natural killer (NK) cells are early responders in bacterial infections but their role in bovine mastitis has not been characterized. For the first time, we show the presence of NK cells (NKp46+/CD3−) in bovine mammary gland tissue after an intramammary challenge with Escherichia (E.) coli. A small number of NK cells was detected in milk from quarters before and during an E. coli challenge. In vitro cultures of primary bovine mammary gland epithelial cells stimulated with UV irradiated E. coli induced significant migration of peripheral blood NK cells (pbNK) within 2 h. Furthermore, pbNK cells significantly reduced counts of live E. coli in vitro within 2 h of culture. The results show that bovine NK cells have the capacity to migrate to the site of infection and produce antibacterial mediators. These findings introduce NK cells as a leukocyte population in the mammary gland with potential functions in the innate immune response in bovine mastitis.",
keywords = "Bovine mastitis, E. coli, Innate immunity, Mammary gland epithelial cells, NK cells",
author = "Anja Sipka and Brianna Pomeroy and Suzanne Klaessig and Ynte Schukken",
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Bovine natural killer cells are present in Escherichia coli infected mammary gland tissue and show antimicrobial activity in vitro. / Sipka, Anja; Pomeroy, Brianna; Klaessig, Suzanne; Schukken, Ynte.

In: Comparative Immunology Microbiology and Infectious Diseases, Vol. 48, 2016, p. 54-60.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Bovine natural killer cells are present in Escherichia coli infected mammary gland tissue and show antimicrobial activity in vitro

AU - Sipka, Anja

AU - Pomeroy, Brianna

AU - Klaessig, Suzanne

AU - Schukken, Ynte

PY - 2016

Y1 - 2016

N2 - Natural killer (NK) cells are early responders in bacterial infections but their role in bovine mastitis has not been characterized. For the first time, we show the presence of NK cells (NKp46+/CD3−) in bovine mammary gland tissue after an intramammary challenge with Escherichia (E.) coli. A small number of NK cells was detected in milk from quarters before and during an E. coli challenge. In vitro cultures of primary bovine mammary gland epithelial cells stimulated with UV irradiated E. coli induced significant migration of peripheral blood NK cells (pbNK) within 2 h. Furthermore, pbNK cells significantly reduced counts of live E. coli in vitro within 2 h of culture. The results show that bovine NK cells have the capacity to migrate to the site of infection and produce antibacterial mediators. These findings introduce NK cells as a leukocyte population in the mammary gland with potential functions in the innate immune response in bovine mastitis.

AB - Natural killer (NK) cells are early responders in bacterial infections but their role in bovine mastitis has not been characterized. For the first time, we show the presence of NK cells (NKp46+/CD3−) in bovine mammary gland tissue after an intramammary challenge with Escherichia (E.) coli. A small number of NK cells was detected in milk from quarters before and during an E. coli challenge. In vitro cultures of primary bovine mammary gland epithelial cells stimulated with UV irradiated E. coli induced significant migration of peripheral blood NK cells (pbNK) within 2 h. Furthermore, pbNK cells significantly reduced counts of live E. coli in vitro within 2 h of culture. The results show that bovine NK cells have the capacity to migrate to the site of infection and produce antibacterial mediators. These findings introduce NK cells as a leukocyte population in the mammary gland with potential functions in the innate immune response in bovine mastitis.

KW - Bovine mastitis

KW - E. coli

KW - Innate immunity

KW - Mammary gland epithelial cells

KW - NK cells

U2 - 10.1016/j.cimid.2016.08.001

DO - 10.1016/j.cimid.2016.08.001

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JO - Comparative Immunology Microbiology and Infectious Diseases

JF - Comparative Immunology Microbiology and Infectious Diseases

SN - 0147-9571

ER -