Bovine lactoferrin enhances TLR7-mediated responses in plasmacytoid dendritic cells in elderly women: Results from a nutritional intervention study with bovine lactoferrin, GOS and Vitamin D

Marloes van Splunter, Olaf Perdijk, Henriëtte Fick-Brinkhof, Anouk L. Feitsma, Esther G. Floris-Vollenbroek, Ben Meijer, Sylvia Brugman, Huub F.J. Savelkoul, Els van Hoffen, R.J.J. van Neerven

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

During aging the immune system is dysregulated. Especially plasmacytoid dendritic cells (pDCs) and myeloid DCs (mDCs) have reduced Toll like receptor (TLR)-mediated responses resulting in increased susceptibility to infections. Consumption of bovine lactoferrin (bLF) has been shown to reduce infections with viruses. Galacto-oligosacharides (GOS) and Vitamin D are associated with reduced pro-inflammatory cytokine levels in serum, and increased TLR7/8 responses, respectively. A double-blind placebo-controlled nutritional intervention study in elderly women was performed, to investigate the potential of bLF, GOS, and Vitamin D to restore TLR responsiveness of pDCs and mDCs and to reduce inflammatory markers in serum. The nutritional intervention group (n = 15) received bLF for 3 weeks, followed by 3 weeks of bLF + GOS, and subsequently 3 weeks of bLF + GOS + Vitamin D. The placebo group (n = 15) received maltodextrin for 9 weeks. Every 3 weeks, blood was collected and TLR responses of pDCs and mDCs, and inflammation-related markers in serum were measured. After 3 weeks of bLF supplementation, increased TLR7/8 and TLR1/2 responses were observed in pDCs of the nutritional intervention group compared to the placebo group. When the effects of the entire nutritional intervention were investigated, increased TLR1/2 mediated responses in mDCs were observed, and in serum sVCAM tended to decrease. Finally, based on the RAND-36 questionnaire physical function tended to improve in the intervention group. Since especially TLR7-mediated responses in pDCs were enhanced after bLF supplementation compared to placebo, this suggests that bLF may contribute to antiviral responses mediated by pDC in elderly women.

LanguageEnglish
Article number2677
JournalFrontiers in Immunology
Volume9
DOIs
Publication statusPublished - 20 Nov 2018

Fingerprint

Lactoferrin
Vitamin D
Dendritic Cells
Toll-Like Receptors
Placebos
Biomarkers
Virus Diseases
Serum
Antiviral Agents
Immune System
Cytokines
Inflammation
Infection

Keywords

  • aging
  • bovine lactoferrin
  • GOS
  • inflammation
  • mDCs
  • pDCs
  • TLR stimulation
  • Vitamin D

Cite this

@article{5269093ac8c7400e876d1b408613b066,
title = "Bovine lactoferrin enhances TLR7-mediated responses in plasmacytoid dendritic cells in elderly women: Results from a nutritional intervention study with bovine lactoferrin, GOS and Vitamin D",
abstract = "During aging the immune system is dysregulated. Especially plasmacytoid dendritic cells (pDCs) and myeloid DCs (mDCs) have reduced Toll like receptor (TLR)-mediated responses resulting in increased susceptibility to infections. Consumption of bovine lactoferrin (bLF) has been shown to reduce infections with viruses. Galacto-oligosacharides (GOS) and Vitamin D are associated with reduced pro-inflammatory cytokine levels in serum, and increased TLR7/8 responses, respectively. A double-blind placebo-controlled nutritional intervention study in elderly women was performed, to investigate the potential of bLF, GOS, and Vitamin D to restore TLR responsiveness of pDCs and mDCs and to reduce inflammatory markers in serum. The nutritional intervention group (n = 15) received bLF for 3 weeks, followed by 3 weeks of bLF + GOS, and subsequently 3 weeks of bLF + GOS + Vitamin D. The placebo group (n = 15) received maltodextrin for 9 weeks. Every 3 weeks, blood was collected and TLR responses of pDCs and mDCs, and inflammation-related markers in serum were measured. After 3 weeks of bLF supplementation, increased TLR7/8 and TLR1/2 responses were observed in pDCs of the nutritional intervention group compared to the placebo group. When the effects of the entire nutritional intervention were investigated, increased TLR1/2 mediated responses in mDCs were observed, and in serum sVCAM tended to decrease. Finally, based on the RAND-36 questionnaire physical function tended to improve in the intervention group. Since especially TLR7-mediated responses in pDCs were enhanced after bLF supplementation compared to placebo, this suggests that bLF may contribute to antiviral responses mediated by pDC in elderly women.",
keywords = "aging, bovine lactoferrin, GOS, inflammation, mDCs, pDCs, TLR stimulation, Vitamin D",
author = "{van Splunter}, Marloes and Olaf Perdijk and Henri{\"e}tte Fick-Brinkhof and Feitsma, {Anouk L.} and Floris-Vollenbroek, {Esther G.} and Ben Meijer and Sylvia Brugman and Savelkoul, {Huub F.J.} and {van Hoffen}, Els and {van Neerven}, R.J.J.",
year = "2018",
month = "11",
day = "20",
doi = "10.3389/fimmu.2018.02677",
language = "English",
volume = "9",
journal = "Frontiers in Immunology",
issn = "1664-3224",
publisher = "Frontiers",

}

Bovine lactoferrin enhances TLR7-mediated responses in plasmacytoid dendritic cells in elderly women : Results from a nutritional intervention study with bovine lactoferrin, GOS and Vitamin D. / van Splunter, Marloes; Perdijk, Olaf; Fick-Brinkhof, Henriëtte; Feitsma, Anouk L.; Floris-Vollenbroek, Esther G.; Meijer, Ben; Brugman, Sylvia; Savelkoul, Huub F.J.; van Hoffen, Els; van Neerven, R.J.J.

In: Frontiers in Immunology, Vol. 9, 2677, 20.11.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Bovine lactoferrin enhances TLR7-mediated responses in plasmacytoid dendritic cells in elderly women

T2 - Frontiers in Immunology

AU - van Splunter, Marloes

AU - Perdijk, Olaf

AU - Fick-Brinkhof, Henriëtte

AU - Feitsma, Anouk L.

AU - Floris-Vollenbroek, Esther G.

AU - Meijer, Ben

AU - Brugman, Sylvia

AU - Savelkoul, Huub F.J.

AU - van Hoffen, Els

AU - van Neerven, R.J.J.

PY - 2018/11/20

Y1 - 2018/11/20

N2 - During aging the immune system is dysregulated. Especially plasmacytoid dendritic cells (pDCs) and myeloid DCs (mDCs) have reduced Toll like receptor (TLR)-mediated responses resulting in increased susceptibility to infections. Consumption of bovine lactoferrin (bLF) has been shown to reduce infections with viruses. Galacto-oligosacharides (GOS) and Vitamin D are associated with reduced pro-inflammatory cytokine levels in serum, and increased TLR7/8 responses, respectively. A double-blind placebo-controlled nutritional intervention study in elderly women was performed, to investigate the potential of bLF, GOS, and Vitamin D to restore TLR responsiveness of pDCs and mDCs and to reduce inflammatory markers in serum. The nutritional intervention group (n = 15) received bLF for 3 weeks, followed by 3 weeks of bLF + GOS, and subsequently 3 weeks of bLF + GOS + Vitamin D. The placebo group (n = 15) received maltodextrin for 9 weeks. Every 3 weeks, blood was collected and TLR responses of pDCs and mDCs, and inflammation-related markers in serum were measured. After 3 weeks of bLF supplementation, increased TLR7/8 and TLR1/2 responses were observed in pDCs of the nutritional intervention group compared to the placebo group. When the effects of the entire nutritional intervention were investigated, increased TLR1/2 mediated responses in mDCs were observed, and in serum sVCAM tended to decrease. Finally, based on the RAND-36 questionnaire physical function tended to improve in the intervention group. Since especially TLR7-mediated responses in pDCs were enhanced after bLF supplementation compared to placebo, this suggests that bLF may contribute to antiviral responses mediated by pDC in elderly women.

AB - During aging the immune system is dysregulated. Especially plasmacytoid dendritic cells (pDCs) and myeloid DCs (mDCs) have reduced Toll like receptor (TLR)-mediated responses resulting in increased susceptibility to infections. Consumption of bovine lactoferrin (bLF) has been shown to reduce infections with viruses. Galacto-oligosacharides (GOS) and Vitamin D are associated with reduced pro-inflammatory cytokine levels in serum, and increased TLR7/8 responses, respectively. A double-blind placebo-controlled nutritional intervention study in elderly women was performed, to investigate the potential of bLF, GOS, and Vitamin D to restore TLR responsiveness of pDCs and mDCs and to reduce inflammatory markers in serum. The nutritional intervention group (n = 15) received bLF for 3 weeks, followed by 3 weeks of bLF + GOS, and subsequently 3 weeks of bLF + GOS + Vitamin D. The placebo group (n = 15) received maltodextrin for 9 weeks. Every 3 weeks, blood was collected and TLR responses of pDCs and mDCs, and inflammation-related markers in serum were measured. After 3 weeks of bLF supplementation, increased TLR7/8 and TLR1/2 responses were observed in pDCs of the nutritional intervention group compared to the placebo group. When the effects of the entire nutritional intervention were investigated, increased TLR1/2 mediated responses in mDCs were observed, and in serum sVCAM tended to decrease. Finally, based on the RAND-36 questionnaire physical function tended to improve in the intervention group. Since especially TLR7-mediated responses in pDCs were enhanced after bLF supplementation compared to placebo, this suggests that bLF may contribute to antiviral responses mediated by pDC in elderly women.

KW - aging

KW - bovine lactoferrin

KW - GOS

KW - inflammation

KW - mDCs

KW - pDCs

KW - TLR stimulation

KW - Vitamin D

U2 - 10.3389/fimmu.2018.02677

DO - 10.3389/fimmu.2018.02677

M3 - Article

VL - 9

JO - Frontiers in Immunology

JF - Frontiers in Immunology

SN - 1664-3224

M1 - 2677

ER -