Bovine herpesvirus 1 interferes with TAP-dependent peptide transport and intracellular trafficking of MHC class I molecules in human cells

D. Koppers-Lalic, M. Rychlowski, D. Leeuwen, F.A.M. Rijsewijk, M.E. Ressing, J.J. Neefjes, K. Bienkowska-Szewczyk, E. Wiertz

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    Abstract

    Bovine herpesvirus 1 (BoHV-1), the cause of infectious bovine rhinotracheitis and infectious pustular vulvovaginitis in cattle, establishes a lifelong infection, despite the presence of antiviral immunity in the host. BoHV-1 has been shown to elude the host immune system, but the viral gene products responsible for this interference have not yet been identified. Studies aiming at the identification of BoHV-1-encoded immune evasion genes have been hampered by the lack of bovine-specific immunological reagents. Some of the immune evasion molecules identified for other herpesviruses are host species specific; others can act across the species barrier. In this study, experiments were performed to investigate whether BoHV-1 can infect human cells and interfere with antigen processing and presentation in these cells. A human melanoma cell line, Mel JuSo, appeared to be permissive for BoHV-1 infection. BoHV-1 induced expression of major viral glycoproteins at the surface of these cells and produced progeny virus up to 105 plaque forming units per ml. BoHV-1 infection resulted in impaired intracellular transport of human MHC class I molecules and inhibition of human TAP. These data indicate that the BoHV-1-encoded molecule(s) that block antigen presentation in bovine cells are able to interact with homologous components of the human MHC class I presentation pathway. The fact that immune evasion by BoHV-1 can be studied in human cells will facilitate the identification of the BoHV-1 gene products involved in this process. Moreover, the data presented here suggest that the BoHV-1 encoded inhibitors of antigen presentation represent potential immune suppressive agents for use in humans.
    Original languageEnglish
    Pages (from-to)2023-2037
    JournalArchives of Virology
    Volume148
    Issue number10
    DOIs
    Publication statusPublished - 2003

    Keywords

    • inhibits antigen presentation
    • regulates surface expression
    • newly synthesized proteins
    • human cytomegalovirus us3
    • simplex-virus icp47
    • bovine herpesvirus-1
    • heavy-chains
    • murine cytomegalovirus
    • endoplasmic-reticulum
    • latent infection

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    Koppers-Lalic, D., Rychlowski, M., Leeuwen, D., Rijsewijk, F. A. M., Ressing, M. E., Neefjes, J. J., ... Wiertz, E. (2003). Bovine herpesvirus 1 interferes with TAP-dependent peptide transport and intracellular trafficking of MHC class I molecules in human cells. Archives of Virology, 148(10), 2023-2037. https://doi.org/10.1007/s00705-003-0142-5