Botryococcus braunii strains compared for biomass productivity, hydrocarbon and carbohydrate content

J.D.G. Gouveia, Jesus Ruiz, Ben van den Broek, T. Hesselink, S.A. Peters, D.M.M. Kleinegris, Alison G. Smith, Douwe van der Veen, M.J. Barbosa, R.H. Wijffels

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Botryococcus braunii can produce both long-chain hydrocarbons as well as carbohydrates in large quantities, and is therefore a promising industrial organism for the production of biopolymer building blocks. Many studies describe the use of different strains of Botryococcus braunii but differences in handling and cultivation conditions make the comparison between strains difficult. In this study, 16 B. braunii strains obtained from six culture collections were compared for their biomass productivity and hydrocarbon and carbohydrate content. Biomass productivity was highest for AC768 strain with 1.8 g L−1 day−1, while hydrocarbon production ranged from none to up to 42% per gram biomass dry weight, with Showa showing the highest hydrocarbon content followed by AC761. The total carbohydrate content varied from 20% to 76% per gram of the biomass dry weight, with CCALA777 as the highest producer. Glucose and galactose are the main monosaccharides in most strains and fucose content reached 463 mg L−1 in CCALA778.
LanguageEnglish
Pages77-86
JournalJournal of Biotechnology
Volume248
DOIs
Publication statusPublished - 2017

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Carbohydrates
Hydrocarbons
Biomass
Productivity
Weights and Measures
Biopolymers
Fucose
Monosaccharides
Galactose
Glucose

Cite this

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title = "Botryococcus braunii strains compared for biomass productivity, hydrocarbon and carbohydrate content",
abstract = "Botryococcus braunii can produce both long-chain hydrocarbons as well as carbohydrates in large quantities, and is therefore a promising industrial organism for the production of biopolymer building blocks. Many studies describe the use of different strains of Botryococcus braunii but differences in handling and cultivation conditions make the comparison between strains difficult. In this study, 16 B. braunii strains obtained from six culture collections were compared for their biomass productivity and hydrocarbon and carbohydrate content. Biomass productivity was highest for AC768 strain with 1.8 g L−1 day−1, while hydrocarbon production ranged from none to up to 42{\%} per gram biomass dry weight, with Showa showing the highest hydrocarbon content followed by AC761. The total carbohydrate content varied from 20{\%} to 76{\%} per gram of the biomass dry weight, with CCALA777 as the highest producer. Glucose and galactose are the main monosaccharides in most strains and fucose content reached 463 mg L−1 in CCALA778.",
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Botryococcus braunii strains compared for biomass productivity, hydrocarbon and carbohydrate content. / Gouveia, J.D.G.; Ruiz, Jesus; van den Broek, Ben; Hesselink, T.; Peters, S.A.; Kleinegris, D.M.M.; Smith, Alison G.; van der Veen, Douwe; Barbosa, M.J.; Wijffels, R.H.

In: Journal of Biotechnology, Vol. 248, 2017, p. 77-86.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Botryococcus braunii strains compared for biomass productivity, hydrocarbon and carbohydrate content

AU - Gouveia, J.D.G.

AU - Ruiz, Jesus

AU - van den Broek, Ben

AU - Hesselink, T.

AU - Peters, S.A.

AU - Kleinegris, D.M.M.

AU - Smith, Alison G.

AU - van der Veen, Douwe

AU - Barbosa, M.J.

AU - Wijffels, R.H.

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AB - Botryococcus braunii can produce both long-chain hydrocarbons as well as carbohydrates in large quantities, and is therefore a promising industrial organism for the production of biopolymer building blocks. Many studies describe the use of different strains of Botryococcus braunii but differences in handling and cultivation conditions make the comparison between strains difficult. In this study, 16 B. braunii strains obtained from six culture collections were compared for their biomass productivity and hydrocarbon and carbohydrate content. Biomass productivity was highest for AC768 strain with 1.8 g L−1 day−1, while hydrocarbon production ranged from none to up to 42% per gram biomass dry weight, with Showa showing the highest hydrocarbon content followed by AC761. The total carbohydrate content varied from 20% to 76% per gram of the biomass dry weight, with CCALA777 as the highest producer. Glucose and galactose are the main monosaccharides in most strains and fucose content reached 463 mg L−1 in CCALA778.

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