Bollworm responses to release of genetically modified Helicoverpa armigera nucleopolyhedroviruses in cotton

X. Sun, X. Chen, Z. Zhang, H. Wang, F.J.J.A. Bianchi, H. Peng, J.M. Vlak, Z. Hu

Research output: Contribution to journalArticleAcademicpeer-review

48 Citations (Scopus)

Abstract

Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) has been developed as a commercial biopesticide to control the cotton bollworm, H. armigera, in China. The major limitation to a broader application of this virus has been the relative long time to incapacitate the target insect. Two HaSNPV recombinants with improved insecticidal properties were released in bollworm-infested cotton. One recombinant (HaCXW1) lacked the ecdysteroid UDP-glucosyltransferase (egt) gene and in another recombinant (HaCXW2), an insect-selective scorpion toxin (AaIT) gene replaced the egt gene. In a cotton field situation H. armigera larvae treated with either HaCXW1 or HaCXW2 were killed faster than larvae in HaSNPV-wt treated plots. Second instar H. armigera larvae, which were collected from HaCXW1 and HaCXW2 treated plots and further reared on artificial diet, showed reduced ST50 values of 15.3 and 26.3°respectively, as compared to larvae collected from HaSNPV-wt treated plots. The reduction in consumed leaf area of field collected larvae infected with HaCXW1 and HaCXW2 was approximated 50 and 63°respectively, as compared to HaSNPV-wt infected larvae at 108 h after treatment. These results suggest that in a cotton field situation the recombinants will be more effective control agents of the cotton bollworm than wild-type HaSNPV
Original languageEnglish
Pages (from-to)63-69
JournalJournal of Invertebrate Pathology
Volume81
DOIs
Publication statusPublished - 2002

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