Blood cell transcriptomics as a source of biomarkers of articular health

J. Sánchez, M.L. Bonet, J. Keijer, E.M. van Schothorst, I. Mölller, C. Chetrit, D. Martinez-Puig, A. Palou

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Background and objectives: We aimed to explore the feasibility of using total human blood RNA as a source of biomarkers of articular health improvement related to glycosaminoglycan (GAG) intake. Methods: Healthy individuals with joint discomfort were enrolled in a prospective nutritional randomized, doubleblind, placebo-controlled study. The supplemented group ate a yoghurt containing MobileeTM - a preparation containing hyaluronic acid (65%) and other GAGs from rooster comb. The effect of Mobilee intake was evaluated in terms of functional and quality-of-life parameters. Whole-genome microarray analysis of blood samples from a subset of 20 subjects collected pre and post intervention was assessed. Results: The daily supplementation with Mobilee significantly reduced pain intensity as compared to placebo. 165 known genes were differentially expressed in blood cells between Mobilee and placebo groups post-intervention, but not pre-intervention (p1.2). Some of them are related to GAG metabolism and extracellular matrix dynamics. In particular, a lower expression of glucurodinase-beta, matrix metallopeptidase 23B (MMP23B), xylosyltransferase II and heparan sulphate 6-O-sulfotransferase 1 (HS6ST1) was found in the Mobilee group. Correlation analysis indicated that the higher the expression of MMP23, involved in the breakdown of extracellular matrix, the higher the pain intensity, and the higher the expression of HS6ST1, responsible for 6-O-sulfation of heparan sulfate, the lower the indicators of knee muscular strength. Conclusions: Genes related to GAG metabolism and extracellular matrix dynamics are differentially expressed in blood cells between the supplement and placebo groups post-intervention, and the expression of some of these genes correlated with indicators of articular pain and muscular strength. Thus, expression levels of specific genes in blood cells could be used as biomarkers of therapeutic benefit of preparations that promotes articular health.
Original languageEnglish
Title of host publicationAbstracts 20th International Congress of Nutrition
Place of PublicationBasel
PublisherKarger
Pages538-538
Volume63
ISBN (Print)9783318025163
Publication statusPublished - 2013
Event20th International Congress of Nutrition, Granada, Spain -
Duration: 15 Sep 201320 Sep 2013

Conference

Conference20th International Congress of Nutrition, Granada, Spain
Period15/09/1320/09/13

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