Black spot partial resistance in diploid roses: QTL discovery and linkage map creation

M. Yan, D.H. Byrne, P.E. Klein, W.E. van de Weg, J. Yang, L. Cai

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Black spot disease (Diplocarpon rosae) is the most important leaf disease of garden roses in warm humid areas. Although partial resistance to black spot has been shown to be moderately heritable, the responsible quantitative trait loci (QTL) remain unidentified. Because of the interspecific nature and high heterozygosity in commercial roses, as well as the relatively small research input compared to row crops, the genomic resources available for rose are limited. To effectively identify markers associated with QTL controlling black spot resistance, abundant markers across the genome and careful phenotyping are required. Fifteen inter-related diploid rose populations with black spot resistant cultivar R. wichuraiana ‘Basye’s Thornless’ in the genetic background were assessed based on the percent of total foliage covered with lesions in June, October and November of 2016 in College Station. Broad sense heritability was estimated at 0.51 which indicates black spot partial resistance is a moderately heritable trait. Genotyping-by-sequencing technology was used to generate SNP markers for linkage map construction. Previous anchor SSR markers were used to designate the linkage group number. The final consensus map used for black spot QTL detection contained 791 SNP covering 430 cM with the biggest gap being 6.6 cM on LG4. One major black spot QTL was discovered on LG3 explaining ~13% of the total phenotypic variance (equaling approximately 26% of the genetic variation) using pedigree-based analysis among all fifteen populations.

LanguageEnglish
Pages135-141
JournalActa Horticulturae
Volume1232
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

chromosome mapping
quantitative trait loci
Rosa
diploidy
Diplocarpon rosae
lesions (plant)
foliar diseases
linkage groups
phenotypic variation
pedigree
genetic background
genotyping
gardens
heterozygosity
heritability
genomics
phenotype
genetic variation
genome
cultivars

Keywords

  • Consensus map
  • Genotyping-by-sequencing
  • Phenotyping
  • QTL
  • SNP

Cite this

Yan, M. ; Byrne, D.H. ; Klein, P.E. ; van de Weg, W.E. ; Yang, J. ; Cai, L. / Black spot partial resistance in diploid roses : QTL discovery and linkage map creation. In: Acta Horticulturae. 2019 ; Vol. 1232. pp. 135-141.
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Black spot partial resistance in diploid roses : QTL discovery and linkage map creation. / Yan, M.; Byrne, D.H.; Klein, P.E.; van de Weg, W.E.; Yang, J.; Cai, L.

In: Acta Horticulturae, Vol. 1232, 01.01.2019, p. 135-141.

Research output: Contribution to journalArticleAcademicpeer-review

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