Biosynthesis of Antinutritional Alkaloids in Solanaceous Crops Is Mediated by Clustered Genes

M. Itkin, U. Heinig, O. Tzfadia, A.J. Bhide, B. Shinde, P.D. Cardenas, S.E. Bocobza, T. Unger, S. Malitsky, H.J. Finkers, Y.M. Tikunov, A.G. Bovy, Y. Chikate, P. Singh, I. Rogachev, J. Beekwilder, A.P. Giri, A. Aharoni

Research output: Contribution to journalArticleAcademicpeer-review

189 Citations (Scopus)

Abstract

Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants—as, for example, potato—are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.
LanguageEnglish
Pages175-179
JournalScience
Volume341
Issue number6142
DOIs
Publication statusPublished - 2013

Fingerprint

glycoalkaloids
alkaloids
biosynthesis
crops
tomatoes
potatoes
genes
solanine
chromosomes
antinutritional factors
food crops
food plants
biochemical pathways
tubers
cholesterol
genomics
fruits

Keywords

  • glycoalkaloids
  • potato
  • plant
  • metabolites
  • pathways
  • saponins
  • tomato

Cite this

Itkin, M., Heinig, U., Tzfadia, O., Bhide, A. J., Shinde, B., Cardenas, P. D., ... Aharoni, A. (2013). Biosynthesis of Antinutritional Alkaloids in Solanaceous Crops Is Mediated by Clustered Genes. Science, 341(6142), 175-179. https://doi.org/10.1126/science.1240230
Itkin, M. ; Heinig, U. ; Tzfadia, O. ; Bhide, A.J. ; Shinde, B. ; Cardenas, P.D. ; Bocobza, S.E. ; Unger, T. ; Malitsky, S. ; Finkers, H.J. ; Tikunov, Y.M. ; Bovy, A.G. ; Chikate, Y. ; Singh, P. ; Rogachev, I. ; Beekwilder, J. ; Giri, A.P. ; Aharoni, A. / Biosynthesis of Antinutritional Alkaloids in Solanaceous Crops Is Mediated by Clustered Genes. In: Science. 2013 ; Vol. 341, No. 6142. pp. 175-179.
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title = "Biosynthesis of Antinutritional Alkaloids in Solanaceous Crops Is Mediated by Clustered Genes",
abstract = "Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants—as, for example, potato—are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.",
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author = "M. Itkin and U. Heinig and O. Tzfadia and A.J. Bhide and B. Shinde and P.D. Cardenas and S.E. Bocobza and T. Unger and S. Malitsky and H.J. Finkers and Y.M. Tikunov and A.G. Bovy and Y. Chikate and P. Singh and I. Rogachev and J. Beekwilder and A.P. Giri and A. Aharoni",
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Itkin, M, Heinig, U, Tzfadia, O, Bhide, AJ, Shinde, B, Cardenas, PD, Bocobza, SE, Unger, T, Malitsky, S, Finkers, HJ, Tikunov, YM, Bovy, AG, Chikate, Y, Singh, P, Rogachev, I, Beekwilder, J, Giri, AP & Aharoni, A 2013, 'Biosynthesis of Antinutritional Alkaloids in Solanaceous Crops Is Mediated by Clustered Genes', Science, vol. 341, no. 6142, pp. 175-179. https://doi.org/10.1126/science.1240230

Biosynthesis of Antinutritional Alkaloids in Solanaceous Crops Is Mediated by Clustered Genes. / Itkin, M.; Heinig, U.; Tzfadia, O.; Bhide, A.J.; Shinde, B.; Cardenas, P.D.; Bocobza, S.E.; Unger, T.; Malitsky, S.; Finkers, H.J.; Tikunov, Y.M.; Bovy, A.G.; Chikate, Y.; Singh, P.; Rogachev, I.; Beekwilder, J.; Giri, A.P.; Aharoni, A.

In: Science, Vol. 341, No. 6142, 2013, p. 175-179.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Biosynthesis of Antinutritional Alkaloids in Solanaceous Crops Is Mediated by Clustered Genes

AU - Itkin, M.

AU - Heinig, U.

AU - Tzfadia, O.

AU - Bhide, A.J.

AU - Shinde, B.

AU - Cardenas, P.D.

AU - Bocobza, S.E.

AU - Unger, T.

AU - Malitsky, S.

AU - Finkers, H.J.

AU - Tikunov, Y.M.

AU - Bovy, A.G.

AU - Chikate, Y.

AU - Singh, P.

AU - Rogachev, I.

AU - Beekwilder, J.

AU - Giri, A.P.

AU - Aharoni, A.

PY - 2013

Y1 - 2013

N2 - Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants—as, for example, potato—are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

AB - Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants—as, for example, potato—are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

KW - glycoalkaloids

KW - potato

KW - plant

KW - metabolites

KW - pathways

KW - saponins

KW - tomato

U2 - 10.1126/science.1240230

DO - 10.1126/science.1240230

M3 - Article

VL - 341

SP - 175

EP - 179

JO - Science

T2 - Science

JF - Science

SN - 0036-8075

IS - 6142

ER -

Itkin M, Heinig U, Tzfadia O, Bhide AJ, Shinde B, Cardenas PD et al. Biosynthesis of Antinutritional Alkaloids in Solanaceous Crops Is Mediated by Clustered Genes. Science. 2013;341(6142):175-179. https://doi.org/10.1126/science.1240230