Biosynthesis and transport of terpenes

H.M. Ting

Research output: Thesisinternal PhD, WU

Abstract

Terpenoids are the largest class of natural product that are produced by plants, with functions that range from a role in plant development to direct defence against pathogens and indirect defence against insects through the attraction of natural enemies. While terpene biosynthesis genes have been well studied, there is still only limited knowledge on how terpenes are transported within the cell and from the cell to the apoplast. In this thesis, different aspects of transport of terpenes in plants were addressed. Firstly, the issue of intermediate transport between enzymes was studied, focussing on the regulation of intermediate flux between two different biosynthesis enzymes (CYP71AV1 and DBR2) that determines the resulting Artemisia annua, low or high artemisinin, chemotype. We also investigated the role of Lipid Transfer Proteins and vesicles in the transport of terpenes. Some LTP mutants were indeed shown to emit lower terpene levels, but the exact mechanism could not be resolved. Inhibition of vesicle transport increased terpene levels, most likely due to an effect on protein stability. I conclude that there are multiple transport mechanisms involved in terpene transport which complicates the analysis of a single transport pathway.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Bouwmeester, Harro, Promotor
  • van der Krol, Sander, Co-promotor
Award date24 Mar 2014
Place of PublicationWageningen
Publisher
Print ISBNs9789461738929
Publication statusPublished - 2014

Keywords

  • nicotiana benthamiana
  • artemisia annua
  • plants
  • plant composition
  • terpenoids
  • biosynthesis
  • genes
  • gene isolation

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