Biomarker development for recovery from stress in pig muscles

M.F.W. te Pas, E. Keuning, L. Kruijt, D.F.M. van de Wiel, J.F. Young, N. Oksbjerg

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Pork quality is negatively influenced by stress due to pre-slaughter exhaustion of energy metabolism components in muscle fibres. Changes in the proteome are expected due to related protein breakdown. Thus proteome profiles may indicate the status of stress and recovery from stress of muscle tissue. Therefore, pigs were exercised on a treadmill for 30 minutes and slaughtered immediately or after a recovery period of 1 or 3 hours. Control pigs were treated as regular slaughter pigs. Each group consisted of 10 pigs. The longissimus and biceps femoris muscles were sampled. Proteomics profiles were determined for each individual sample using the SELDI-TOF-MS equipment and 3 different array types analyzing positively or negatively charged proteins, or phosphorylated proteins, respectively. Analyses were performed with the software provided with the equipment. The analyses compared the expression levels of individual peaks for each of the animals per muscle and per treatment group. All proteomics experiments were done in duplicate. Each proteomics experiment was individually analyzed and only peaks that appeared in both analyses were considered significant. The first results indicate that the changes of the expression of a number of peaks may be related to stress and recovery of stress. Compared to non-stressed control pigs the expression of these proteins is either decreased or elevated after stress and the changes are reversed during the recovery period. However, peaks differ in the timing of this process. Therefore, at present we cannot conclude on the association between peak expression profile and stress recovery. Further analyses on the repeatability of the analyses are ongoing. Finally, it may be necessary to associate the proteomics expression profiles with physiological indicators of muscle response to stress before conclusions about possible biomarkers can be done.
Original languageEnglish
Title of host publicationBook of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009
PublisherWageningen Academic Publishers
Pages279-279
ISBN (Print)9789086861217
Publication statusPublished - 2009
Event60th Annual Meeting of the European Association for Animal Production -
Duration: 24 Aug 200927 Aug 2009

Conference

Conference60th Annual Meeting of the European Association for Animal Production
Period24/08/0927/08/09

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