Biological nitrogen fixation of soybean in acid soils of Sumatra, Indonesia

S.H. Waluyo

Research output: Thesisinternal PhD, WU


<p>The aim of this study is to improve soybean cultivation in transmigration areas, especially in Sitiung, West Sumatra. However, these soils are very acid, and have a high P-fixing capacity. To reduce the amounts of fertilisers, normally 5 - 7 ton lime ha <sup>-1</sup> and 100 kg P as TSP, seed, pelleted with lime (60 kg ha <sup>-1</sup> ) and TSP (10 kg ha <sup>-1</sup> ), was introduced. In this way only 2 ton lime ha <sup>-1</sup> are required.</p><p>Soybean can fix nitrogen (BNF) in symbiosis with ( <em>Brady</em> ) <em>Rhizobium</em> bacteria. However, these acid soils in general, have low numbers of ( <em>Brady</em> ) <em>Rhizobium</em> . By inoculating the soils with ( <em>Brady</em> ) <em>Rhizobium</em> , BNF of soybean, and yield, were considerably improved.</p><p>A study was made of the indigenous ( <em>Brady</em> ) <em>Rhizobium</em> population in view of the following:</p><ul><lem>Although at the beginning the numbers may be low, by repeated soybean cultivation, the numbers will increase, and they may interfere with inoculation of effective ( <em>Brady</em> ) <em>Rhizobium</em> strains.</lem><lem>These indigenous ( <em>Brady</em> ) <em>Rhizobium</em> are adapted to local stress conditions, and they may be useful for the improvement of strains, to be used as inoculants.</lem></ul><p>Using molecular techniques, indigenous strains derived from soil samples from old soybean areas (Java) and from new soybean areas (Sumatra) were classified in more detail. Most likely <em>B. japonicum</em> is the dominant strain in Java while in Sumatra <em>B. elkanii</em> is more present. A <em>Sinorhizobium fredii</em> -like strain was isolated from one soil sample from Java.</p>
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • de Vos, W.M., Promotor
  • 't Mannetje, L., Promotor, External person
  • An, L.T., Promotor, External person
Award date6 Nov 2000
Place of PublicationS.l.
Print ISBNs9789058082954
Publication statusPublished - 2000


  • glycine max
  • soyabeans
  • soil biology
  • nitrogen fixation
  • nitrogen fixing bacteria
  • rhizobium
  • bradyrhizobium
  • inoculation
  • inoculum
  • biochemical techniques
  • dna fingerprinting
  • strain differences
  • strains
  • seed treatment
  • pelleting
  • acid soils
  • soil acidity
  • liming
  • sumatra
  • indonesia


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