Biohydrogen Production from Glycerol using Thermotoga spp

B.T. Maru, A.A.M. Bielen, S.W.M. Kengen, M. Constantini, F. Medina

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademicpeer-review

23 Citations (Scopus)


Given the highly reduced state of carbon in glycerol and its availability as a substantial byproduct of biodiesel production, glycerol is of special interest for sustainable biofuel production. Glycerol was used as a substrate for biohydrogen production using the hyperthermophilic bacterium, Thermotoga maritima and Thermotoga neapolitana. Both species metabolized glycerol to mainly acetate and hydrogen. At glycerol concentrations of 2.5 g/L, hydrogen was produced with a yield of 2.75 and 2.65 mol H2/mol glycerol consumed by T. maritima and T. neapolitana respectively. Additionally, the effect of initial pH (ranging between pH 5.0-8.5) and yeast extract concentrations (0.5, 1, 2, 4 g/L) on glycerol fermentation by T. neapolitana was investigated in batch systems. An initial pH value of around 7 was optimal for hydrogen production by T. neapolitana. Lower concentration of yeast extract resulted in a lower H2 production, however increasing the concentration from 2 to 4 g/L did not affect H2 production
Original languageEnglish
Title of host publicationWHEC 2012 Conference Proceedings - 19th World Hydrogen Energy Conference, 03-07 June 2012, Toronto, Canada
Publication statusPublished - 2012
Event19th World Hydrogen Energy Conference, Toronto, Canada -
Duration: 3 Jun 20127 Jun 2012


Conference19th World Hydrogen Energy Conference, Toronto, Canada

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