Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity

Emmanuelle Charpentier*, Hagen Richter, John van der Oost, Malcolm F. White

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

123 Citations (Scopus)

Abstract

CRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key elements in the interference step of the immune pathway. A CRISPR array composed of a series of repeats interspaced by spacer sequences acquired from invading mobile genomes is transcribed as a precursor crRNA (pre-crRNA) molecule. This pre-crRNA undergoes one or two maturation steps to generate the mature crRNAs that guide CRISPR-associated (Cas) protein(s) to cognate invading genomes for their destruction. Different types of CRISPR-Cas systems have evolved distinct crRNA biogenesis pathways that implicate highly sophisticated processing mechanisms. In Types I and III CRISPR-Cas systems, a specific endoribonuclease of the Cas6 family, either standalone or in a complex with other Cas proteins, cleaves the pre-crRNA within the repeat regions. In Type II systems, the trans-acting small RNA (tracrRNA) base pairs with each repeat of the pre-crRNA to form a dual-RNA that is cleaved by the housekeeping RNase III in the presence of the protein Cas9. In this review, we present a detailed comparative analysis of pre-crRNA recognition and cleavage mechanisms involved in the biogenesis of guide crRNAs in the three CRISPR-Cas types.

Original languageEnglish
Pages (from-to)428-441
JournalFEMS Microbiology Reviews
Volume39
Issue number3
DOIs
Publication statusPublished - 2015

Keywords

  • Cas5d
  • Cas6
  • Cas9
  • crRNA biogenesis
  • RNase III
  • TracrRNA

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