The importance of Southeast Asia’s tropical peat swamp forests for biodiversity is becoming increasingly recognised. Information on species presence within peatland areas is scant, however, limiting our ability to develop species conservation strategies and monitor responses to human activities. We compile species presence records for the Sebangau forest in Indonesian Borneo since 1993 and present the most complete Bornean PSF biodiversity inventory yet published. Including morpho-species that are likely to represent true species, this list comprises 215 tree, 92 non-tree flora, 73 ant, 66 butterfly, 297 spider, 41 dragon/damselfly, 55 fish, 11 amphibian, 46 reptile, 172 bird and 65 mammal taxa. Of these, 46 species are globally threatened and 59 are currently protected in Indonesia; 22 vertebrate species are Borneo endemics. Because our sampling is both biased and incomplete, the true number of species found at this site is likely to be much higher. Little is known about many of these taxa in Sebangau and peat swamp forests elsewhere. Many of these species are considered forest dependent, and the entire community is expected to be important for maintaining the resilience of the peat swamp forest ecosystem and the environmental services that it provides. This highlights the need for urgent conservation of Sebangau and its diverse biological community.