Biodegradation pathway of L-glutamatediacetate by Rhizobium radiobacter strain BG-1

C.G. van Ginkel, R. Geerts, P.D. Nguyen, C.M. Plugge

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

An aerobic bacterium was isolated from activated sludge in a medium containing l-glutamate-N,N-diacetate (l-GLDA) as sole carbon and energy source. The isolate was identified as a Rhizobium radiobacter species. Besides l-GLDA, the strain utilized nitrilotriacetate (NTA) and proposed intermediates in l-GLDA metabolism such as glyoxylate and l-glutamate. l-GLDA-grown cells oxidized l-GLDA, l-glutamate but not iminodiacetate (IDA), and trans-ketoglutaconate, indicating removal of a carboxymethyl group as an initial degradation reaction. The removal of the first carboxymethyl group of l-GLDA is catalyzed by an NADH-dependent mono-oxygenase. The oxidative deamination of l-glutamate by a dehydrogenase resulting in the formation of oxoglutarate was also detected in cell-free extracts of R. radiobacter sp. A pathway for the metabolism of l-GLDA R. radiobacter sp. is proposed: First, l-GLDA leads to l-glutamate-N-monoacetate (l-GLMA) which in turn leads to l-glutamate. Then, l-glutamate leads to oxoglutarate, an intermediate of the TCA cycle.
Original languageEnglish
Pages (from-to)31-37
JournalInternational Biodeterioration and Biodegradation
Volume62
Issue number1
DOIs
Publication statusPublished - 2008

Keywords

  • chelatobacter-heintzii
  • agrobacterium sp
  • edta
  • degradation
  • iminodisuccinate
  • purification
  • identification
  • stereoisomers
  • dehydrogenase
  • bacterium

Fingerprint Dive into the research topics of 'Biodegradation pathway of L-glutamatediacetate by Rhizobium radiobacter strain BG-1'. Together they form a unique fingerprint.

Cite this