Biochip spray: simplified coupling of surface plasmon resonance biosensing and mass spectrometry

S. Joshi, H. Zuilhof, T.A. van Beek, M.W.F. Nielen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

A simplified coupling of surface plasmon resonance (SPR) immuno-biosensing with ambient ionization mass spectrometry (MS) was developed. It combines two orthogonal analysis techniques: the biosensing capability of SPR and the chemical identification power of high resolution MS. As a proof-of-principle, deoxynivalenol (DON), an important mycotoxin, was captured using an SPR gold chip containing an antifouling layer and monoclonal antibodies against the toxin and, after washing, the chip could be taken out and analyzed by direct spray MS of the biosensor chip to confirm the identity of DON. Furthermore, cross-reacting conjugates of DON present in a naturally contaminated beer could be successfully identified, thus showing the potential of rapid identification of (un)expected cross-reacting molecules.
Original languageEnglish
Pages (from-to)1427-1432
JournalAnalytical Chemistry
Volume89
Issue number3
DOIs
Publication statusPublished - 2017

Fingerprint Dive into the research topics of 'Biochip spray: simplified coupling of surface plasmon resonance biosensing and mass spectrometry'. Together they form a unique fingerprint.

  • Projects

    Cite this