Biochip spray: simplified coupling of surface plasmon resonance biosensing and mass spectrometry

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14 Citations (Scopus)

Abstract

A simplified coupling of surface plasmon resonance (SPR) immuno-biosensing with ambient ionization mass spectrometry (MS) was developed. It combines two orthogonal analysis techniques: the biosensing capability of SPR and the chemical identification power of high resolution MS. As a proof-of-principle, deoxynivalenol (DON), an important mycotoxin, was captured using an SPR gold chip containing an antifouling layer and monoclonal antibodies against the toxin and, after washing, the chip could be taken out and analyzed by direct spray MS of the biosensor chip to confirm the identity of DON. Furthermore, cross-reacting conjugates of DON present in a naturally contaminated beer could be successfully identified, thus showing the potential of rapid identification of (un)expected cross-reacting molecules.
LanguageEnglish
Pages1427-1432
JournalAnalytical Chemistry
Volume89
Issue number3
DOIs
Publication statusPublished - 2017

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Biochips
Surface plasmon resonance
Mass spectrometry
Beer
Mycotoxins
Biosensors
Washing
Gold
Ionization
Monoclonal Antibodies
Molecules
deoxynivalenol

Cite this

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title = "Biochip spray: simplified coupling of surface plasmon resonance biosensing and mass spectrometry",
abstract = "A simplified coupling of surface plasmon resonance (SPR) immuno-biosensing with ambient ionization mass spectrometry (MS) was developed. It combines two orthogonal analysis techniques: the biosensing capability of SPR and the chemical identification power of high resolution MS. As a proof-of-principle, deoxynivalenol (DON), an important mycotoxin, was captured using an SPR gold chip containing an antifouling layer and monoclonal antibodies against the toxin and, after washing, the chip could be taken out and analyzed by direct spray MS of the biosensor chip to confirm the identity of DON. Furthermore, cross-reacting conjugates of DON present in a naturally contaminated beer could be successfully identified, thus showing the potential of rapid identification of (un)expected cross-reacting molecules.",
author = "S. Joshi and H. Zuilhof and {van Beek}, T.A. and M.W.F. Nielen",
year = "2017",
doi = "10.1021/acs.analchem.6b04012",
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Biochip spray: simplified coupling of surface plasmon resonance biosensing and mass spectrometry. / Joshi, S.; Zuilhof, H.; van Beek, T.A.; Nielen, M.W.F.

In: Analytical Chemistry, Vol. 89, No. 3, 2017, p. 1427-1432.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Biochip spray: simplified coupling of surface plasmon resonance biosensing and mass spectrometry

AU - Joshi, S.

AU - Zuilhof, H.

AU - van Beek, T.A.

AU - Nielen, M.W.F.

PY - 2017

Y1 - 2017

N2 - A simplified coupling of surface plasmon resonance (SPR) immuno-biosensing with ambient ionization mass spectrometry (MS) was developed. It combines two orthogonal analysis techniques: the biosensing capability of SPR and the chemical identification power of high resolution MS. As a proof-of-principle, deoxynivalenol (DON), an important mycotoxin, was captured using an SPR gold chip containing an antifouling layer and monoclonal antibodies against the toxin and, after washing, the chip could be taken out and analyzed by direct spray MS of the biosensor chip to confirm the identity of DON. Furthermore, cross-reacting conjugates of DON present in a naturally contaminated beer could be successfully identified, thus showing the potential of rapid identification of (un)expected cross-reacting molecules.

AB - A simplified coupling of surface plasmon resonance (SPR) immuno-biosensing with ambient ionization mass spectrometry (MS) was developed. It combines two orthogonal analysis techniques: the biosensing capability of SPR and the chemical identification power of high resolution MS. As a proof-of-principle, deoxynivalenol (DON), an important mycotoxin, was captured using an SPR gold chip containing an antifouling layer and monoclonal antibodies against the toxin and, after washing, the chip could be taken out and analyzed by direct spray MS of the biosensor chip to confirm the identity of DON. Furthermore, cross-reacting conjugates of DON present in a naturally contaminated beer could be successfully identified, thus showing the potential of rapid identification of (un)expected cross-reacting molecules.

U2 - 10.1021/acs.analchem.6b04012

DO - 10.1021/acs.analchem.6b04012

M3 - Article

VL - 89

SP - 1427

EP - 1432

JO - Analytical Chemistry

T2 - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 3

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