Bioavailibility of selenium from fish, yeast and selenate: a comparative study in humans using stable isotopes

T.E. Fox, E.G.H.M. van den Heuvel, C.A. Atherton, J.B. Luten, M. van Hoek-van Nieuwenhuizen, M.J.J. Kotterman

    Research output: Contribution to journalArticleAcademicpeer-review

    83 Citations (Scopus)


    Objective: To measure the bioavailability of selenium from cooked and raw fish in humans by estimating and comparing apparent absorption and retention of selenium in biosynthetically labelled fish with labelled selenate and biosynthetically labelled selenium in brewers yeast. Design: The intervention study was a parallel, randomised, reference substance controlled design carried out at two different centres in Europe. Setting: The human study was carried out at the Institute of Food Research, Norwich, UK and at TNO Nutrition and Food Research, Zeist, The Netherlands. Subjects: In all, 35 male volunteers aged 18-50 y were recruited; 17 subjects were studied in Norwich (UK) and 18 in Zeist (Netherlands). All of the recruited subjects completed the study. Interventions: Biosynthetically labelled trout fish (processed by two different methods), biosynthetically labelled brewers yeast and isotopically labelled selenate were used to estimate selenium apparent absorption and retention by quantitative analysis of stable isotope labels recovered in faeces and urine. Subjects consumed the labelled foods in four meals over two consecutive days and absorption was measured by the luminal disappearance method over 10 days. Urinary clearance of isotopic labels was measured over 7 days to enable retention to be calculated. Results: Apparent absorption of selenium from fish was similar to selenate and there was no difference between the two processing methods used. However, retention of fish selenium was significantly higher than selenate (P
    Original languageEnglish
    Pages (from-to)343-349
    JournalEuropean Journal of Clinical Nutrition
    Publication statusPublished - 2004


    • plasma-mass spectrometry
    • new-zealand women
    • glutathione-peroxidase
    • trace-elements
    • human health
    • selenomethionine
    • metabolism
    • chromatography
    • blood
    • selenoproteins

    Fingerprint Dive into the research topics of 'Bioavailibility of selenium from fish, yeast and selenate: a comparative study in humans using stable isotopes'. Together they form a unique fingerprint.

  • Cite this