Bioavailability of genistein, daidzein, and their glycosides in intestinal epithelial Caco-2 cells

A. Steensma, H.P.J.M. Noteborn, R.C.M. van der Jagt, Th.H.G. Polman, M.J.B. Mengelers, H.A. Kuiper

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Abstract

In this study information was obtained on bioavailability of genistein, daidzein and their glycosides in human intestinal epithelial Caco-2 cells grown on semi-permeable filters. The integrity of Caco-2 monolayers was confirmed by transepithelial electrical resistance measurements and by determination of the permeability of the radioactive marker polyethylene glycol (PEG4000). After 6 h approximately 30–40% of genistein and daidzein added at the apical side was transported to the basolateral side and this level was maintained for 24 h, The glycosides were barely transported through the Caco-2 cells. No significant metabolism of genistein and daidzein in the Caco-2 cells occurred, whereas the glycosides were mainly metabolised to their respective aglycones. Obviously, our data indicates that Caco-2 cells contain an endogenous glycosidase activity.
Original languageEnglish
Pages (from-to)209-212
JournalEnvironmental Toxicology and Pharmacology
Volume7
DOIs
Publication statusPublished - 1999

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    Steensma, A., Noteborn, H. P. J. M., van der Jagt, R. C. M., Polman, T. H. G., Mengelers, M. J. B., & Kuiper, H. A. (1999). Bioavailability of genistein, daidzein, and their glycosides in intestinal epithelial Caco-2 cells. Environmental Toxicology and Pharmacology, 7, 209-212. https://doi.org/10.1016/S1382-6689(99)00015-0