Bioavailability of genistein, daidzein, and their glycosides in intestinal epithelial Caco-2 cells

A. Steensma; H.P.J.M. Noteborn; R.C.M. van der Jagt; Th.H.G. Polman; M.J.B. Mengelers; H.A. Kuiper

doi:10.1016/S1382-6689(99)00015-0

Bioavailability of genistein, daidzein, and their glycosides in intestinal epithelial Caco-2 cells

A. Steensma, H.P.J.M. Noteborn, R.C.M. van der Jagt, Th.H.G. Polman, M.J.B. Mengelers, H.A. Kuiper

Research output: Contribution to journal › Article › Academic › peer-review

32 Citations (Scopus)

Abstract

In this study information was obtained on bioavailability of genistein, daidzein and their glycosides in human intestinal epithelial Caco-2 cells grown on semi-permeable filters. The integrity of Caco-2 monolayers was confirmed by transepithelial electrical resistance measurements and by determination of the permeability of the radioactive marker polyethylene glycol (PEG4000). After 6 h approximately 30–40% of genistein and daidzein added at the apical side was transported to the basolateral side and this level was maintained for 24 h, The glycosides were barely transported through the Caco-2 cells. No significant metabolism of genistein and daidzein in the Caco-2 cells occurred, whereas the glycosides were mainly metabolised to their respective aglycones. Obviously, our data indicates that Caco-2 cells contain an endogenous glycosidase activity.

Original language	English
Pages (from-to)	209-212
Journal	Environmental Toxicology and Pharmacology
Volume	7
DOIs	https://doi.org/10.1016/S1382-6689(99)00015-0
Publication status	Published - 1999

Access to Document

10.1016/S1382-6689(99)00015-0

Fingerprint Dive into the research topics of 'Bioavailability of genistein, daidzein, and their glycosides in intestinal epithelial Caco-2 cells'. Together they form a unique fingerprint.

Cite this

@article{84555f3c26ba441285a311e9032c0072,

title = "Bioavailability of genistein, daidzein, and their glycosides in intestinal epithelial Caco-2 cells",

abstract = "In this study information was obtained on bioavailability of genistein, daidzein and their glycosides in human intestinal epithelial Caco-2 cells grown on semi-permeable filters. The integrity of Caco-2 monolayers was confirmed by transepithelial electrical resistance measurements and by determination of the permeability of the radioactive marker polyethylene glycol (PEG4000). After 6 h approximately 30–40% of genistein and daidzein added at the apical side was transported to the basolateral side and this level was maintained for 24 h, The glycosides were barely transported through the Caco-2 cells. No significant metabolism of genistein and daidzein in the Caco-2 cells occurred, whereas the glycosides were mainly metabolised to their respective aglycones. Obviously, our data indicates that Caco-2 cells contain an endogenous glycosidase activity.",

author = "A. Steensma and H.P.J.M. Noteborn and {van der Jagt}, R.C.M. and Th.H.G. Polman and M.J.B. Mengelers and H.A. Kuiper",

year = "1999",

doi = "10.1016/S1382-6689(99)00015-0",

language = "English",

volume = "7",

pages = "209--212",

journal = "Environmental Toxicology and Pharmacology",

issn = "1382-6689",

publisher = "Elsevier",

}

TY - JOUR

T1 - Bioavailability of genistein, daidzein, and their glycosides in intestinal epithelial Caco-2 cells

AU - Steensma, A.

AU - Noteborn, H.P.J.M.

AU - van der Jagt, R.C.M.

AU - Polman, Th.H.G.

AU - Mengelers, M.J.B.

AU - Kuiper, H.A.

PY - 1999

Y1 - 1999

N2 - In this study information was obtained on bioavailability of genistein, daidzein and their glycosides in human intestinal epithelial Caco-2 cells grown on semi-permeable filters. The integrity of Caco-2 monolayers was confirmed by transepithelial electrical resistance measurements and by determination of the permeability of the radioactive marker polyethylene glycol (PEG4000). After 6 h approximately 30–40% of genistein and daidzein added at the apical side was transported to the basolateral side and this level was maintained for 24 h, The glycosides were barely transported through the Caco-2 cells. No significant metabolism of genistein and daidzein in the Caco-2 cells occurred, whereas the glycosides were mainly metabolised to their respective aglycones. Obviously, our data indicates that Caco-2 cells contain an endogenous glycosidase activity.

AB - In this study information was obtained on bioavailability of genistein, daidzein and their glycosides in human intestinal epithelial Caco-2 cells grown on semi-permeable filters. The integrity of Caco-2 monolayers was confirmed by transepithelial electrical resistance measurements and by determination of the permeability of the radioactive marker polyethylene glycol (PEG4000). After 6 h approximately 30–40% of genistein and daidzein added at the apical side was transported to the basolateral side and this level was maintained for 24 h, The glycosides were barely transported through the Caco-2 cells. No significant metabolism of genistein and daidzein in the Caco-2 cells occurred, whereas the glycosides were mainly metabolised to their respective aglycones. Obviously, our data indicates that Caco-2 cells contain an endogenous glycosidase activity.

U2 - 10.1016/S1382-6689(99)00015-0

DO - 10.1016/S1382-6689(99)00015-0

M3 - Article

VL - 7

SP - 209

EP - 212

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

SN - 1382-6689

ER -