Bioactive lipopeptides of ice-nucleating snow bacterium Pseudomonas syringae strain 31R1

Alberto Fiore, Luisa Mannina, Anatoli P. Sobolev, Anna Maria Salzano, Andrea Scaloni, Ingeborg Grgurina, Maria Rosaria Fullone, Monica Gallo, Camille Swasey, Vincenzo Fogliano, Jon Y. Takemoto

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

The production of secondary metabolite lipopeptides by ice-nucleating Pseudomonas syringae strain 31R1 was investigated. Pseudomonas syringae strain 31R1 is a rifampicin-resistant derivative of P. syringae no. 31 used for the commercial production of snow. It is shown that P. syringae strain 31R1 produces antifungal lipodepsipeptides, syringomycins E and G, and, in addition, a novel and unique lipopeptide, peptin31. Spectroscopic and spectrometric analyses revealed that peptin31 is a linear undecalipopeptide with sequence identities to N- and C-terminal portions but lacking 11 amino acids of known lipodepsipeptide syringopeptin SPPhv. Peptin31 displayed antifungal activities against Rhodotorula pilimanae, Rhizoctonia solani, and Trichoderma harzianum and also hemolytic and antibacterial activities. Extracts of P. syringae strain 31R1 grown in medium with chloride were fungicidal, but not when grown without chloride. The latter extracts lacked peptin 31 and contained des-chloro forms of syringomycins E and G with low antifungal activities. Thus, the three lipopeptides account for the fungicidal properties of P. syringae 31R1 extracts. The occurrence of these bioactive metabolites should be considered when P. syringae no. 31 and its derivatives are used in products for making artificial snow.

Original languageEnglish
Pages (from-to)158-165
Number of pages8
JournalFEMS Microbiology Letters
Volume286
Issue number2
DOIs
Publication statusPublished - 1 Sep 2008
Externally publishedYes

Keywords

  • Fungicidal
  • Lipodepsipeptide
  • Pseudomonas syringae
  • Undecalipopeptide

Fingerprint Dive into the research topics of 'Bioactive lipopeptides of ice-nucleating snow bacterium Pseudomonas syringae strain 31R1'. Together they form a unique fingerprint.

Cite this