Bioactive Antifouling Surfaces by Visible-Light-Triggered Polymerization

Andriy R. Kuzmyn, Ai T. Nguyen, Han Zuilhof*, Jacob Baggerman

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Hierarchical bioactive surfaces are created by visible-light-induced surface-initiated living radical polymerization employing tris[2-phenylpyridinato-C2,N]iridium(III) as a photocatalyst. The hierarchical antifouling diblock copolymer structures consist of N-(2-hydroxypropyl)-methacrylamide (first block) and carboxybetaine methacrylate (second block). The living nature of the polymerization is shown by a linear increase in layer thickness (as measured by atomic force microscopy) and reinitiation of the polymerization to create a patterned second block of polymer. The chemical structure of the brushes is confirmed by X-ray photoelectron spectroscopy and attenuated total reflection Fourier transform infrared spectroscopy measurements. The block copolymer brushes demonstrate excellent antifouling properties when exposed to single-protein solutions or to bovine serum. The second carboxybetaine block of the hierarchical antifouling structures can effectively be biofunctionalized with an anti-fibrinogen antibody. The coated surfaces show a high affinity and specificity to fibrinogen, while preventing nonspecific adsorption from other proteins in bovine serum.

Original languageEnglish
Article number1900351
JournalAdvanced Materials Interfaces
Volume6
Issue number12
Early online date2 May 2019
DOIs
Publication statusPublished - 21 Jun 2019

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Polymerization
Brushes
Block copolymers
Proteins
Living polymerization
Iridium
Photocatalysts
Free radical polymerization
Antibodies
Fourier transform infrared spectroscopy
Atomic force microscopy
X ray photoelectron spectroscopy
Adsorption
Polymers

Keywords

  • biosensing
  • controlled living polymerization
  • nonfouling coatings
  • zwitterionic polymer brushes

Cite this

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title = "Bioactive Antifouling Surfaces by Visible-Light-Triggered Polymerization",
abstract = "Hierarchical bioactive surfaces are created by visible-light-induced surface-initiated living radical polymerization employing tris[2-phenylpyridinato-C2,N]iridium(III) as a photocatalyst. The hierarchical antifouling diblock copolymer structures consist of N-(2-hydroxypropyl)-methacrylamide (first block) and carboxybetaine methacrylate (second block). The living nature of the polymerization is shown by a linear increase in layer thickness (as measured by atomic force microscopy) and reinitiation of the polymerization to create a patterned second block of polymer. The chemical structure of the brushes is confirmed by X-ray photoelectron spectroscopy and attenuated total reflection Fourier transform infrared spectroscopy measurements. The block copolymer brushes demonstrate excellent antifouling properties when exposed to single-protein solutions or to bovine serum. The second carboxybetaine block of the hierarchical antifouling structures can effectively be biofunctionalized with an anti-fibrinogen antibody. The coated surfaces show a high affinity and specificity to fibrinogen, while preventing nonspecific adsorption from other proteins in bovine serum.",
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month = "6",
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language = "English",
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Bioactive Antifouling Surfaces by Visible-Light-Triggered Polymerization. / Kuzmyn, Andriy R.; Nguyen, Ai T.; Zuilhof, Han; Baggerman, Jacob.

In: Advanced Materials Interfaces, Vol. 6, No. 12, 1900351, 21.06.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Bioactive Antifouling Surfaces by Visible-Light-Triggered Polymerization

AU - Kuzmyn, Andriy R.

AU - Nguyen, Ai T.

AU - Zuilhof, Han

AU - Baggerman, Jacob

PY - 2019/6/21

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N2 - Hierarchical bioactive surfaces are created by visible-light-induced surface-initiated living radical polymerization employing tris[2-phenylpyridinato-C2,N]iridium(III) as a photocatalyst. The hierarchical antifouling diblock copolymer structures consist of N-(2-hydroxypropyl)-methacrylamide (first block) and carboxybetaine methacrylate (second block). The living nature of the polymerization is shown by a linear increase in layer thickness (as measured by atomic force microscopy) and reinitiation of the polymerization to create a patterned second block of polymer. The chemical structure of the brushes is confirmed by X-ray photoelectron spectroscopy and attenuated total reflection Fourier transform infrared spectroscopy measurements. The block copolymer brushes demonstrate excellent antifouling properties when exposed to single-protein solutions or to bovine serum. The second carboxybetaine block of the hierarchical antifouling structures can effectively be biofunctionalized with an anti-fibrinogen antibody. The coated surfaces show a high affinity and specificity to fibrinogen, while preventing nonspecific adsorption from other proteins in bovine serum.

AB - Hierarchical bioactive surfaces are created by visible-light-induced surface-initiated living radical polymerization employing tris[2-phenylpyridinato-C2,N]iridium(III) as a photocatalyst. The hierarchical antifouling diblock copolymer structures consist of N-(2-hydroxypropyl)-methacrylamide (first block) and carboxybetaine methacrylate (second block). The living nature of the polymerization is shown by a linear increase in layer thickness (as measured by atomic force microscopy) and reinitiation of the polymerization to create a patterned second block of polymer. The chemical structure of the brushes is confirmed by X-ray photoelectron spectroscopy and attenuated total reflection Fourier transform infrared spectroscopy measurements. The block copolymer brushes demonstrate excellent antifouling properties when exposed to single-protein solutions or to bovine serum. The second carboxybetaine block of the hierarchical antifouling structures can effectively be biofunctionalized with an anti-fibrinogen antibody. The coated surfaces show a high affinity and specificity to fibrinogen, while preventing nonspecific adsorption from other proteins in bovine serum.

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KW - controlled living polymerization

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KW - zwitterionic polymer brushes

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