Binning metagenomic contigs by coverage and composition

J. Alneberg, B.S. Bjarnason, I. de Bruijn, M. Schirmer, J. Quick, U.Z. Ijaz, L.M. Lahti, N.J. Loman, A.F. Andersson, C. Quince

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412 Citations (Scopus)

Abstract

Shotgun sequencing enables the reconstruction of genomes from complex microbial communities, but because assembly does not reconstruct entire genomes, it is necessary to bin genome fragments. Here we present CONCOCT, a new algorithm that combines sequence composition and coverage across multiple samples, to automatically cluster contigs into genomes. We demonstrate high recall and precision on artificial as well as real human gut metagenome data sets.
Original languageEnglish
Pages (from-to)1144-1146
JournalNature Methods : techniques for life scientists and chemists
Volume11
DOIs
Publication statusPublished - 2014

Keywords

  • sequences
  • genomes

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    Alneberg, J., Bjarnason, B. S., de Bruijn, I., Schirmer, M., Quick, J., Ijaz, U. Z., Lahti, L. M., Loman, N. J., Andersson, A. F., & Quince, C. (2014). Binning metagenomic contigs by coverage and composition. Nature Methods : techniques for life scientists and chemists, 11, 1144-1146. https://doi.org/10.1038/nmeth.3103