Binding of the CrylVD toxin of Bacillus thuringiensis subsp. israelensis to larval dipteran midgut proteins

    Research output: Contribution to journalArticleAcademicpeer-review

    23 Citations (Scopus)

    Abstract

    Ligand-blotting experiments on dipteran brush border membrane vesicles (BBMVs) showed binding of CryIVD toxin of Bacillus thuringiensis subsp. israelensis to proteins of 148 kDa in Anopheles stephensi and of 78 kDa in Tipula oleracea, both species being susceptible to CryIVD. Binding of CryIVD with BBMVs of A. stephensi resulted in a stronger signal than with BBMVs of T. oleracea. Likewise, larvae of A. stephensi are 10,000-fold more susceptible to the CryIVD toxin than are larvae of T. oleracea. Binding was also found with six proteins ranging in size from 48 to 110 kDa in BBMVs from the lepidopteran species Manduca sexta, but CryIVD was not toxic for M. sexta larvae. No binding of trypsinated CryIVD to BBMV proteins was observed. With the lepidopteran-specific toxin CryIA(b), no binding to dipteran BBMVs was found. Binding of CryIA(b) to nine different BBMV proteins ranging in size from 71 to 240 kDa was observed in M. sexta. The major binding signal was observed with a protein of 240 kDa for CryIA(b).
    Original languageEnglish
    Pages (from-to)2601-2605
    JournalApplied and Environmental Microbiology
    Volume61
    Issue number7
    Publication statusPublished - 1995

    Fingerprint Dive into the research topics of 'Binding of the CrylVD toxin of Bacillus thuringiensis subsp. israelensis to larval dipteran midgut proteins'. Together they form a unique fingerprint.

  • Cite this