Bilberry xyloglucan - novel building blocks containing ß-xylose within a complex structure

H. Hilz; L.E. de Jong; M.A. Kabel; R.P. Verhoef; H.A. Schols; A.G.J. Voragen

doi:10.1016/j.carres.2006.12.005

Bilberry xyloglucan - novel building blocks containing ß-xylose within a complex structure

H. Hilz, L.E. de Jong, M.A. Kabel, R.P. Verhoef, H.A. Schols, A.G.J. Voragen

Research output: Contribution to journal › Article › Academic › peer-review

37 Citations (Scopus)

Abstract

Bilberries are known to have one of the most complex xyloglucan structures described in the plant kingdom until now. To characterise this structure, xyloglucans were enzymatically degraded and the oligosaccharides obtained were analysed. More than 20 different building blocks were found to make up the xyloglucan polymer. Bilberry xyloglucan was of XXXG-type, but some XXG-type oligomers were present, as well. The building blocks contain galactose¿xylose (L) and fucose¿galactose¿xylose (F) side chains. In both side chains, the galactose units can be acetylated. In addition, ß-xylose-¿-xylose (U) side chains were shown. This U chain was present in three building blocks described before (XUXG, XLUG and XUFG) and four novel blocks that have not been described in the literature previously: XUG, XUUG, XLUG and XXUG

Original language	English
Pages (from-to)	170-181
Journal	Carbohydrate Research : an international journal
Volume	342
Issue number	2
DOIs	https://doi.org/10.1016/j.carres.2006.12.005
Publication status	Published - 2007

Keywords

plant-cell walls
cultured sycamore cells
anion-exchange chromatography
mass-spectrometry
black-currants
cellulose interactions
liquid-chromatography
fruit xyloglucan
oligosaccharides
polysaccharides

Access to Document

10.1016/j.carres.2006.12.005

Cite this

@article{4519ddee423a4a0e93d2a0a3ff50745a,

title = "Bilberry xyloglucan - novel building blocks containing {\ss}-xylose within a complex structure",

abstract = "Bilberries are known to have one of the most complex xyloglucan structures described in the plant kingdom until now. To characterise this structure, xyloglucans were enzymatically degraded and the oligosaccharides obtained were analysed. More than 20 different building blocks were found to make up the xyloglucan polymer. Bilberry xyloglucan was of XXXG-type, but some XXG-type oligomers were present, as well. The building blocks contain galactose¿xylose (L) and fucose¿galactose¿xylose (F) side chains. In both side chains, the galactose units can be acetylated. In addition, {\ss}-xylose-¿-xylose (U) side chains were shown. This U chain was present in three building blocks described before (XUXG, XLUG and XUFG) and four novel blocks that have not been described in the literature previously: XUG, XUUG, XLUG and XXUG",

keywords = "plant-cell walls, cultured sycamore cells, anion-exchange chromatography, mass-spectrometry, black-currants, cellulose interactions, liquid-chromatography, fruit xyloglucan, oligosaccharides, polysaccharides",

author = "H. Hilz and {de Jong}, L.E. and M.A. Kabel and R.P. Verhoef and H.A. Schols and A.G.J. Voragen",

year = "2007",

doi = "10.1016/j.carres.2006.12.005",

language = "English",

volume = "342",

pages = "170--181",

journal = "Carbohydrate Research : an international journal",

issn = "0008-6215",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Bilberry xyloglucan - novel building blocks containing ß-xylose within a complex structure

AU - Hilz, H.

AU - de Jong, L.E.

AU - Kabel, M.A.

AU - Verhoef, R.P.

AU - Schols, H.A.

AU - Voragen, A.G.J.

PY - 2007

Y1 - 2007

N2 - Bilberries are known to have one of the most complex xyloglucan structures described in the plant kingdom until now. To characterise this structure, xyloglucans were enzymatically degraded and the oligosaccharides obtained were analysed. More than 20 different building blocks were found to make up the xyloglucan polymer. Bilberry xyloglucan was of XXXG-type, but some XXG-type oligomers were present, as well. The building blocks contain galactose¿xylose (L) and fucose¿galactose¿xylose (F) side chains. In both side chains, the galactose units can be acetylated. In addition, ß-xylose-¿-xylose (U) side chains were shown. This U chain was present in three building blocks described before (XUXG, XLUG and XUFG) and four novel blocks that have not been described in the literature previously: XUG, XUUG, XLUG and XXUG

AB - Bilberries are known to have one of the most complex xyloglucan structures described in the plant kingdom until now. To characterise this structure, xyloglucans were enzymatically degraded and the oligosaccharides obtained were analysed. More than 20 different building blocks were found to make up the xyloglucan polymer. Bilberry xyloglucan was of XXXG-type, but some XXG-type oligomers were present, as well. The building blocks contain galactose¿xylose (L) and fucose¿galactose¿xylose (F) side chains. In both side chains, the galactose units can be acetylated. In addition, ß-xylose-¿-xylose (U) side chains were shown. This U chain was present in three building blocks described before (XUXG, XLUG and XUFG) and four novel blocks that have not been described in the literature previously: XUG, XUUG, XLUG and XXUG

KW - plant-cell walls

KW - cultured sycamore cells

KW - anion-exchange chromatography

KW - mass-spectrometry

KW - black-currants

KW - cellulose interactions

KW - liquid-chromatography

KW - fruit xyloglucan

KW - oligosaccharides

KW - polysaccharides

U2 - 10.1016/j.carres.2006.12.005

DO - 10.1016/j.carres.2006.12.005

M3 - Article

SN - 0008-6215

VL - 342

SP - 170

EP - 181

JO - Carbohydrate Research : an international journal

JF - Carbohydrate Research : an international journal

IS - 2

ER -

Bilberry xyloglucan - novel building blocks containing ß-xylose within a complex structure

Abstract

Keywords

Access to Document

Fingerprint

Cite this