Bijdrage tot de kennis van de caseinaat phosphaatphase der melk

A critical discussion was given of the extensive literature on the nature of the complex of calcium caseinate phosphate. A series of different experiments were performed to elucidate this complicated problem. It was inferred that calcium bound to casein must be considered separate from the calcium bound to inorganic phosphate. Casein-bound calcium was thought to be linked to the ester phosphate groups of the casein molecule. This contradicted the often assumed structure of double salts, and the author supposed the inorganic phosphate and the remaining calcium to be separately and individually linked to various reactive sites on the casein molecule, mostly amino and carboxyl groups. The relation Ca/P of the adsorbed ions was found to be about 1.5.

On heating milk, according to the author tricalcium phosphate was precipitated from the serum, and subsequently calcium and phosphate were replenished from the caseinate complex. This could go on until all was present in the form of tricalcium phosphate. This could be re-adsorbed as such onto the caseinate complex.