Bifidobacterium carbohydrases-their role in breakdown and synthesis of (potential) prebiotics

Research output: Contribution to journalArticleAcademicpeer-review

104 Citations (Scopus)

Abstract

Abstract There is an increasing interest to positively influence the human intestinal microbiota through the diet by the use of prebiotics and/or probiotics. It is anticipated that this will balance the microbial composition in the gastrointestinal tract in favor of health promoting genera such as Bifidobacterium and Lactobacillus. Carbohydrates like non-digestible oligosaccharides are potential prebiotics. To understand how these bacteria can grow on these carbon sources, knowledge of the carbohydrate-modifying enzymes is needed. Little is known about the carbohydrate-modifying enzymes of bifidobacteria. The genome sequence of Bifidobacterium adolescentis and Bifidobacterium longum biotype longum has been completed and it was observed that for B. longum biotype longum more than 8% of the annotated genes were involved in carbohydrate metabolism. In addition more sequence data of individual carbohydrases from other Bifidobacterium spp. became available. Besides the degradation of (potential) prebiotics by bifidobacterial glycoside hydrolases, we will focus in this review on the possibilities to produce new classes of non-digestible oligosaccharides by showing the presence and (transglycosylation) activity of the most important carbohydrate modifying enzymes in bifidobacteria. Approaches to use and improve carbohydrate-modifying enzymes in prebiotic design will be discussed.
Original languageEnglish
Pages (from-to)146-163
JournalMolecular Nutrition & Food Research
Volume52
Issue number1
DOIs
Publication statusPublished - 2008

Fingerprint

Prebiotics
Bifidobacterium
prebiotics
Carbohydrates
Bifidobacterium longum subsp. longum
carbohydrates
synthesis
Sulfalene
Enzymes
enzymes
Oligosaccharides
oligosaccharides
Bifidobacterium adolescentis
health promotion
Glycoside Hydrolases
Carbohydrate Metabolism
Probiotics
Lactobacillus
carbohydrate metabolism
hydrolases

Keywords

  • adolescentis dsm 20083
  • intestinal anaerobic bacterium
  • sequence-based classification
  • alpha-l-arabinofuranosidase
  • glycoside hydrolase family
  • lactic-acid bacteria
  • beta-d-galactosidase
  • sucrose phosphorylase
  • molecular-cloning
  • escherichia-coli

Cite this

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title = "Bifidobacterium carbohydrases-their role in breakdown and synthesis of (potential) prebiotics",
abstract = "Abstract There is an increasing interest to positively influence the human intestinal microbiota through the diet by the use of prebiotics and/or probiotics. It is anticipated that this will balance the microbial composition in the gastrointestinal tract in favor of health promoting genera such as Bifidobacterium and Lactobacillus. Carbohydrates like non-digestible oligosaccharides are potential prebiotics. To understand how these bacteria can grow on these carbon sources, knowledge of the carbohydrate-modifying enzymes is needed. Little is known about the carbohydrate-modifying enzymes of bifidobacteria. The genome sequence of Bifidobacterium adolescentis and Bifidobacterium longum biotype longum has been completed and it was observed that for B. longum biotype longum more than 8{\%} of the annotated genes were involved in carbohydrate metabolism. In addition more sequence data of individual carbohydrases from other Bifidobacterium spp. became available. Besides the degradation of (potential) prebiotics by bifidobacterial glycoside hydrolases, we will focus in this review on the possibilities to produce new classes of non-digestible oligosaccharides by showing the presence and (transglycosylation) activity of the most important carbohydrate modifying enzymes in bifidobacteria. Approaches to use and improve carbohydrate-modifying enzymes in prebiotic design will be discussed.",
keywords = "adolescentis dsm 20083, intestinal anaerobic bacterium, sequence-based classification, alpha-l-arabinofuranosidase, glycoside hydrolase family, lactic-acid bacteria, beta-d-galactosidase, sucrose phosphorylase, molecular-cloning, escherichia-coli",
author = "{van den Broek}, L.A.M. and S.W.A. Hinz and G. Beldman and J.P. Vincken and A.G.J. Voragen",
year = "2008",
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journal = "Molecular Nutrition & Food Research",
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Bifidobacterium carbohydrases-their role in breakdown and synthesis of (potential) prebiotics. / van den Broek, L.A.M.; Hinz, S.W.A.; Beldman, G.; Vincken, J.P.; Voragen, A.G.J.

In: Molecular Nutrition & Food Research, Vol. 52, No. 1, 2008, p. 146-163.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Bifidobacterium carbohydrases-their role in breakdown and synthesis of (potential) prebiotics

AU - van den Broek, L.A.M.

AU - Hinz, S.W.A.

AU - Beldman, G.

AU - Vincken, J.P.

AU - Voragen, A.G.J.

PY - 2008

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N2 - Abstract There is an increasing interest to positively influence the human intestinal microbiota through the diet by the use of prebiotics and/or probiotics. It is anticipated that this will balance the microbial composition in the gastrointestinal tract in favor of health promoting genera such as Bifidobacterium and Lactobacillus. Carbohydrates like non-digestible oligosaccharides are potential prebiotics. To understand how these bacteria can grow on these carbon sources, knowledge of the carbohydrate-modifying enzymes is needed. Little is known about the carbohydrate-modifying enzymes of bifidobacteria. The genome sequence of Bifidobacterium adolescentis and Bifidobacterium longum biotype longum has been completed and it was observed that for B. longum biotype longum more than 8% of the annotated genes were involved in carbohydrate metabolism. In addition more sequence data of individual carbohydrases from other Bifidobacterium spp. became available. Besides the degradation of (potential) prebiotics by bifidobacterial glycoside hydrolases, we will focus in this review on the possibilities to produce new classes of non-digestible oligosaccharides by showing the presence and (transglycosylation) activity of the most important carbohydrate modifying enzymes in bifidobacteria. Approaches to use and improve carbohydrate-modifying enzymes in prebiotic design will be discussed.

AB - Abstract There is an increasing interest to positively influence the human intestinal microbiota through the diet by the use of prebiotics and/or probiotics. It is anticipated that this will balance the microbial composition in the gastrointestinal tract in favor of health promoting genera such as Bifidobacterium and Lactobacillus. Carbohydrates like non-digestible oligosaccharides are potential prebiotics. To understand how these bacteria can grow on these carbon sources, knowledge of the carbohydrate-modifying enzymes is needed. Little is known about the carbohydrate-modifying enzymes of bifidobacteria. The genome sequence of Bifidobacterium adolescentis and Bifidobacterium longum biotype longum has been completed and it was observed that for B. longum biotype longum more than 8% of the annotated genes were involved in carbohydrate metabolism. In addition more sequence data of individual carbohydrases from other Bifidobacterium spp. became available. Besides the degradation of (potential) prebiotics by bifidobacterial glycoside hydrolases, we will focus in this review on the possibilities to produce new classes of non-digestible oligosaccharides by showing the presence and (transglycosylation) activity of the most important carbohydrate modifying enzymes in bifidobacteria. Approaches to use and improve carbohydrate-modifying enzymes in prebiotic design will be discussed.

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