Bidirectional backcrosses between wild and cultivated lettuce identify loci involved in nonhost resistance to downy mildew

Anne K.J. Giesbers, Erik Den Boer, David N.J. Braspenning, Thijs P.H. Bouten, Johan W. Specken, Martijn P.W. van Kaauwen, Richard G.F. Visser, Rients E. Niks, Marieke J.W. Jeuken*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Key message: The nonhost resistance of wild lettuce to lettuce downy mildew seems explained by four components of a putative set of epistatic genes. Abstract: The commonplace observation that plants are immune to most potential pathogens is known as nonhost resistance (NHR). The genetic basis of NHR is poorly understood. Inheritance studies of NHR require crosses of nonhost species with a host, but these crosses are usually unsuccessful. The plant-pathosystem of lettuce and downy mildew, Bremia lactucae, provides a rare opportunity to study the inheritance of NHR, because the nonhost wild lettuce species Lactuca saligna is sufficiently cross-compatible with the cultivated host Lactuca sativa. Our previous studies on NHR in one L. saligna accession led to the hypothesis that multi-locus epistatic interactions might explain NHR. Here, we studied NHR at the species level in nine accessions. Besides the commonly used approach of studying a target trait from a wild donor species in a cultivar genetic background, we also explored the opposite, complementary approach of cultivar introgression in a wild species background. This bidirectional approach encompassed (1) nonhost into host introgression: identification of L. saligna derived chromosome regions that were overrepresented in highly resistant BC1 plants (F1 × L. sativa), (2) host into nonhost introgression: identification of L. sativa derived chromosome regions that were overrepresented in BC1 inbred lines (F1 × L. saligna) with relatively high infection levels. We demonstrated that NHR is based on resistance factors from L. saligna and the genetic dose for NHR differs between accessions. NHR seemed explained by combinations of epistatic genes on three or four chromosome segments, of which one chromosome segment was validated by the host into nonhost approach.

Original languageEnglish
Pages (from-to)1761-1776
JournalTheoretical and Applied Genetics
Volume131
Issue number8
Early online date25 May 2018
DOIs
Publication statusPublished - Aug 2018

Fingerprint

Lactuca saligna
Lettuce
downy mildew
lettuce
Lactuca sativa
loci
introgression
chromosomes
epistasis
Genetic Epistasis
Chromosomes
inheritance (genetics)
Bremia lactucae
cross resistance
cultivars
genetic background
inbred lines
R Factors
pathogens
dosage

Cite this

@article{2d6dd58f270541a9b7ff97f123261cbe,
title = "Bidirectional backcrosses between wild and cultivated lettuce identify loci involved in nonhost resistance to downy mildew",
abstract = "Key message: The nonhost resistance of wild lettuce to lettuce downy mildew seems explained by four components of a putative set of epistatic genes. Abstract: The commonplace observation that plants are immune to most potential pathogens is known as nonhost resistance (NHR). The genetic basis of NHR is poorly understood. Inheritance studies of NHR require crosses of nonhost species with a host, but these crosses are usually unsuccessful. The plant-pathosystem of lettuce and downy mildew, Bremia lactucae, provides a rare opportunity to study the inheritance of NHR, because the nonhost wild lettuce species Lactuca saligna is sufficiently cross-compatible with the cultivated host Lactuca sativa. Our previous studies on NHR in one L. saligna accession led to the hypothesis that multi-locus epistatic interactions might explain NHR. Here, we studied NHR at the species level in nine accessions. Besides the commonly used approach of studying a target trait from a wild donor species in a cultivar genetic background, we also explored the opposite, complementary approach of cultivar introgression in a wild species background. This bidirectional approach encompassed (1) nonhost into host introgression: identification of L. saligna derived chromosome regions that were overrepresented in highly resistant BC1 plants (F1 × L. sativa), (2) host into nonhost introgression: identification of L. sativa derived chromosome regions that were overrepresented in BC1 inbred lines (F1 × L. saligna) with relatively high infection levels. We demonstrated that NHR is based on resistance factors from L. saligna and the genetic dose for NHR differs between accessions. NHR seemed explained by combinations of epistatic genes on three or four chromosome segments, of which one chromosome segment was validated by the host into nonhost approach.",
author = "Giesbers, {Anne K.J.} and {Den Boer}, Erik and Braspenning, {David N.J.} and Bouten, {Thijs P.H.} and Specken, {Johan W.} and {van Kaauwen}, {Martijn P.W.} and Visser, {Richard G.F.} and Niks, {Rients E.} and Jeuken, {Marieke J.W.}",
year = "2018",
month = "8",
doi = "10.1007/s00122-018-3112-8",
language = "English",
volume = "131",
pages = "1761--1776",
journal = "Theoretical and Applied Genetics",
issn = "0040-5752",
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Bidirectional backcrosses between wild and cultivated lettuce identify loci involved in nonhost resistance to downy mildew. / Giesbers, Anne K.J.; Den Boer, Erik; Braspenning, David N.J.; Bouten, Thijs P.H.; Specken, Johan W.; van Kaauwen, Martijn P.W.; Visser, Richard G.F.; Niks, Rients E.; Jeuken, Marieke J.W.

In: Theoretical and Applied Genetics, Vol. 131, No. 8, 08.2018, p. 1761-1776.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Bidirectional backcrosses between wild and cultivated lettuce identify loci involved in nonhost resistance to downy mildew

AU - Giesbers, Anne K.J.

AU - Den Boer, Erik

AU - Braspenning, David N.J.

AU - Bouten, Thijs P.H.

AU - Specken, Johan W.

AU - van Kaauwen, Martijn P.W.

AU - Visser, Richard G.F.

AU - Niks, Rients E.

AU - Jeuken, Marieke J.W.

PY - 2018/8

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N2 - Key message: The nonhost resistance of wild lettuce to lettuce downy mildew seems explained by four components of a putative set of epistatic genes. Abstract: The commonplace observation that plants are immune to most potential pathogens is known as nonhost resistance (NHR). The genetic basis of NHR is poorly understood. Inheritance studies of NHR require crosses of nonhost species with a host, but these crosses are usually unsuccessful. The plant-pathosystem of lettuce and downy mildew, Bremia lactucae, provides a rare opportunity to study the inheritance of NHR, because the nonhost wild lettuce species Lactuca saligna is sufficiently cross-compatible with the cultivated host Lactuca sativa. Our previous studies on NHR in one L. saligna accession led to the hypothesis that multi-locus epistatic interactions might explain NHR. Here, we studied NHR at the species level in nine accessions. Besides the commonly used approach of studying a target trait from a wild donor species in a cultivar genetic background, we also explored the opposite, complementary approach of cultivar introgression in a wild species background. This bidirectional approach encompassed (1) nonhost into host introgression: identification of L. saligna derived chromosome regions that were overrepresented in highly resistant BC1 plants (F1 × L. sativa), (2) host into nonhost introgression: identification of L. sativa derived chromosome regions that were overrepresented in BC1 inbred lines (F1 × L. saligna) with relatively high infection levels. We demonstrated that NHR is based on resistance factors from L. saligna and the genetic dose for NHR differs between accessions. NHR seemed explained by combinations of epistatic genes on three or four chromosome segments, of which one chromosome segment was validated by the host into nonhost approach.

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M3 - Article

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EP - 1776

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