Molecular mechanisms triggered by high dietary beta-carotene (BC) intake in lung are largely unknown. We performed microarray gene expression analysis on lung tissue of BC supplemented beta-carotene 15,150-monooxygenase 1 knockout (Bcmo1-/-) mice, which are—like humans—able to accumulate BC. Our main observation was that the genes were regulated in an opposite direction in male and female Bcmo1-/- mice by BC. The steroid biosynthetic pathway was overrepresented in BC-supplemented male Bcmo1-/- mice. Testosterone levels were higher after BC supplementation only in Bcmo1-/- mice, which had, unlike wild-type (Bcmo1?/?) mice, large variations. We hypothesize that BC possibly affects hormone synthesis or metabolism. Since sex hormones influence lung cancer risk, these data might contribute to an explanation for the previously found increased lung cancer risk after BC supplementation (ATBC and CARET studies). Moreover, effects of BC may depend on the presence of frequent human BCMO1 polymorphisms, since these effects were not found in wild-type mice.
|Journal||Cellular and Molecular Life Sciences|
|Publication status||Published - 2011|
- nitric-oxide synthase
- base-line characteristics
- retinol efficacy trial
- epidemiologic evidence
- cancer incidence
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Nutritional effects by beta-carotene in lung in males and females of control mice versus BCMO knockout mice
van Schothorst, E. (Creator), Helden, Y. G. J. (Creator), Keijer, J. (Creator), Bunschoten, A. (Creator), von Lintig, J. (Creator) & Lietz, G. (Creator), Wageningen University, 26 Sept 2017
Nutritional effects by beta-carotene versus control in lung, inguinal white adipose tissue, and liver in males and females of control wildtype mice versus BCMO/BCO1 knockout mice
van Schothorst, E. (Creator), Wageningen University, 26 Sept 2017