Abstract
| Language | English |
|---|---|
| Pages | 283-292 |
| Journal | European journal of immunology |
| Volume | 48 |
| Issue number | 2 |
| Early online date | 11 Oct 2017 |
| DOIs | |
| Publication status | Published - 9 Feb 2018 |
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BAFF augments IgA2 and IL-10 production by TLR7/8 stimulated total peripheral blood B cells. / den Hartog, C.G.; van Osch, Thijs L.J.; Vos, Martijn; Meijer, B.; Savelkoul, H.F.J.; van Neerven, R.J.J.; Brugman, S.
In: European journal of immunology, Vol. 48, No. 2, 09.02.2018, p. 283-292.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - BAFF augments IgA2 and IL-10 production by TLR7/8 stimulated total peripheral blood B cells
AU - den Hartog, C.G.
AU - van Osch, Thijs L.J.
AU - Vos, Martijn
AU - Meijer, B.
AU - Savelkoul, H.F.J.
AU - van Neerven, R.J.J.
AU - Brugman, S.
PY - 2018/2/9
Y1 - 2018/2/9
N2 - Class-switching of B cells to IgA can be induced via both T-cell-dependent and T-cell-independent mechanisms. IgA is most predominantly produced mucosally and is important for combating infections and allergies. In contrast to mice, humans have two forms of IgA; IgA1 and IgA2 with diverse tissue distribution. In early life, IgA levels might be sub-optimal especially during the fall season when bacterial and viral infections are more common. Therefore, we investigated using human B cells whether T-cell-independent factors -promoting cell survival, class switching and immunoglobulin secretion- BAFF, APRIL, IL-10 and retinoic acid can boost IgA production in the context of viral or bacterial infection. To this end total and naive peripheral blood B cells were stimulated with these factors for 6 days in the presence or absence of TLR7/8 agonist R848 (mimicking viral infection) or TLR9 agonist CpG-ODN (mimicking bacterial infection). We show that BAFF significantly augments IgA2 production in TLR7/8 stimulated mature, but not naïve B cells. In addition, BAFF augments IL-10 production and viability in TLR7/8 and TLR9 stimulated mature B cells. These data warrant further investigation of its role in immune regulation both in the periphery and mucosal tissues in early life or during disease.
AB - Class-switching of B cells to IgA can be induced via both T-cell-dependent and T-cell-independent mechanisms. IgA is most predominantly produced mucosally and is important for combating infections and allergies. In contrast to mice, humans have two forms of IgA; IgA1 and IgA2 with diverse tissue distribution. In early life, IgA levels might be sub-optimal especially during the fall season when bacterial and viral infections are more common. Therefore, we investigated using human B cells whether T-cell-independent factors -promoting cell survival, class switching and immunoglobulin secretion- BAFF, APRIL, IL-10 and retinoic acid can boost IgA production in the context of viral or bacterial infection. To this end total and naive peripheral blood B cells were stimulated with these factors for 6 days in the presence or absence of TLR7/8 agonist R848 (mimicking viral infection) or TLR9 agonist CpG-ODN (mimicking bacterial infection). We show that BAFF significantly augments IgA2 production in TLR7/8 stimulated mature, but not naïve B cells. In addition, BAFF augments IL-10 production and viability in TLR7/8 and TLR9 stimulated mature B cells. These data warrant further investigation of its role in immune regulation both in the periphery and mucosal tissues in early life or during disease.
U2 - 10.1002/eji.201646861
DO - 10.1002/eji.201646861
M3 - Article
VL - 48
SP - 283
EP - 292
JO - European journal of immunology
T2 - European journal of immunology
JF - European journal of immunology
SN - 0014-2980
IS - 2
ER -