Baculoviruses hijack the visual perception of their caterpillar hosts to induce climbing behaviour thus promoting virus dispersal

Xiaoming Liu, Zhiqiang Tian, Limei Cai, Zhongjian Shen, J.P. Michaud, Lin Zhu, Shuo Yan, Vera I.D. Ros, Kelli Hoover, Zhen Li, Songdou Zhang, Xiaoxia Liu*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Baculoviruses can induce climbing behaviour in their caterpillar hosts to ensure they die at elevated positions to enhance virus transmission, providing an excellent model to study parasitic manipulation of host behaviour. Here, we demonstrate that climbing behaviour occurred mostly during daylight hours, and that the height at death of Helicoverpa armigera single nucleopolyhedrovirus (HearNPV)-infected larvae increases with the height of the light source. Phototaxic and electroretinogram (ERG) responses were enhanced after HearNPV-infection in host larvae, and ablation of stemmata in infected larvae prevented both phototaxis and climbing behaviour. Through transcriptome and quantitative PCR, we confirmed that two opsin genes (a blue light-sensitive gene, HaBL; and a long wave-sensitive gene, HaLW) as well as the TRPL (transient receptor potential-like channel protein) gene, all integral to the host's visual perception pathway, were significantly upregulated after HearNPV infection. Knockout of HaBL, HaLW, or TRPL genes using the CRISPR/Cas9 system resulted in significantly reduced ERG responses, phototaxis, and climbing behaviour in HearNPV-infected larvae. These results reveal that HearNPV alters the expression of specific genes to hijack host visual perception at fundamental levels—photoreception and phototransduction—in order to induce climbing behaviour in host larvae.

Original languageEnglish
Pages (from-to)2752-2765
JournalMolecular Ecology
Volume31
Issue number9
Early online date8 Mar 2022
DOIs
Publication statusPublished - May 2022

Keywords

  • opsin
  • phototaxis
  • transmission

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