Bacterial translocation and in vivo assessment of intestinal barrier permeability in Rainbow trout (Oncorhynchus mykiss) with and without soyabean meal-induced inflammation

Peyman Mosberian Tanha, M. Overland, Thor Landsverk, Felipe E. Reveco, J.W. Schrama, A.J. Roem, Jane W. Agger, Liv T. Midland

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Abstract

The primary aim of this experiment was to evaluate the intestinal barrier permeability in vivo in rainbow trout (Oncorhynchus mykiss) fed increasing levels of soyabean meal (SBM). The relationship between SBM-induced enteritis (SBMIE) and the permeability markers was also investigated. Our results showed that the mean score of morphological parameters was significantly higher as a result of 37·5 % SBM inclusion in the diet, while the scores of fish fed 25 % SBM or lower were not different from those of the fish meal-fed controls (P < 0·05). SBMIE was found in the distal intestine (DI) in 18 % of the fish (eleven of sixty): ten in the 37·5 % SBM-fed group and one in the 25 % SBM-fed group. Sugar markers in plasma showed large variation among individuals probably due to variation in feed intake. We found, however, a significant linear increase in the level of plasma d-lactate with increasing SBM inclusion level (P < 0·0001). Plasma concentration of endotoxin was not significantly different in groups with or without SBMIE. Some individual fish showed high values of endotoxin in blood, but the same individuals did not show any bacterial translocation. Plasma bacterial DNA was detected in 28 % of the fish with SBMIE, and 8 % of non-SBMIE fish (P = 0·07). Plasma concentration of d-lactate was significantly higher in fish with SBMIE (P < 0·0001). To conclude, SBMIE in the DI of rainbow trout was associated with an increase in bacterial translocation and plasma d-lactate concentration, suggesting that these permeability markers can be used to evaluate intestinal permeability in vivo.
Original languageEnglish
Article numbere26
Number of pages10
JournalJournal of Nutritional Science
Volume5
DOIs
Publication statusPublished - 2016

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Bacterial Translocation
Oncorhynchus mykiss
enteritis
Enteritis
soybean meal
Meals
Permeability
permeability
inflammation
Fishes
Inflammation
lactates
fish
Lactic Acid
endotoxins
Endotoxins
intestines
Intestines
Bacterial DNA
fish meal

Cite this

Mosberian Tanha, Peyman ; Overland, M. ; Landsverk, Thor ; Reveco, Felipe E. ; Schrama, J.W. ; Roem, A.J. ; Agger, Jane W. ; Midland, Liv T. / Bacterial translocation and in vivo assessment of intestinal barrier permeability in Rainbow trout (Oncorhynchus mykiss) with and without soyabean meal-induced inflammation. In: Journal of Nutritional Science. 2016 ; Vol. 5 .
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title = "Bacterial translocation and in vivo assessment of intestinal barrier permeability in Rainbow trout (Oncorhynchus mykiss) with and without soyabean meal-induced inflammation",
abstract = "The primary aim of this experiment was to evaluate the intestinal barrier permeability in vivo in rainbow trout (Oncorhynchus mykiss) fed increasing levels of soyabean meal (SBM). The relationship between SBM-induced enteritis (SBMIE) and the permeability markers was also investigated. Our results showed that the mean score of morphological parameters was significantly higher as a result of 37·5 {\%} SBM inclusion in the diet, while the scores of fish fed 25 {\%} SBM or lower were not different from those of the fish meal-fed controls (P < 0·05). SBMIE was found in the distal intestine (DI) in 18 {\%} of the fish (eleven of sixty): ten in the 37·5 {\%} SBM-fed group and one in the 25 {\%} SBM-fed group. Sugar markers in plasma showed large variation among individuals probably due to variation in feed intake. We found, however, a significant linear increase in the level of plasma d-lactate with increasing SBM inclusion level (P < 0·0001). Plasma concentration of endotoxin was not significantly different in groups with or without SBMIE. Some individual fish showed high values of endotoxin in blood, but the same individuals did not show any bacterial translocation. Plasma bacterial DNA was detected in 28 {\%} of the fish with SBMIE, and 8 {\%} of non-SBMIE fish (P = 0·07). Plasma concentration of d-lactate was significantly higher in fish with SBMIE (P < 0·0001). To conclude, SBMIE in the DI of rainbow trout was associated with an increase in bacterial translocation and plasma d-lactate concentration, suggesting that these permeability markers can be used to evaluate intestinal permeability in vivo.",
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author = "{Mosberian Tanha}, Peyman and M. Overland and Thor Landsverk and Reveco, {Felipe E.} and J.W. Schrama and A.J. Roem and Agger, {Jane W.} and Midland, {Liv T.}",
year = "2016",
doi = "10.1017/jns.2016.7",
language = "English",
volume = "5",
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Bacterial translocation and in vivo assessment of intestinal barrier permeability in Rainbow trout (Oncorhynchus mykiss) with and without soyabean meal-induced inflammation. / Mosberian Tanha, Peyman; Overland, M.; Landsverk, Thor; Reveco, Felipe E.; Schrama, J.W.; Roem, A.J.; Agger, Jane W.; Midland, Liv T.

In: Journal of Nutritional Science, Vol. 5 , e26, 2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Bacterial translocation and in vivo assessment of intestinal barrier permeability in Rainbow trout (Oncorhynchus mykiss) with and without soyabean meal-induced inflammation

AU - Mosberian Tanha, Peyman

AU - Overland, M.

AU - Landsverk, Thor

AU - Reveco, Felipe E.

AU - Schrama, J.W.

AU - Roem, A.J.

AU - Agger, Jane W.

AU - Midland, Liv T.

PY - 2016

Y1 - 2016

N2 - The primary aim of this experiment was to evaluate the intestinal barrier permeability in vivo in rainbow trout (Oncorhynchus mykiss) fed increasing levels of soyabean meal (SBM). The relationship between SBM-induced enteritis (SBMIE) and the permeability markers was also investigated. Our results showed that the mean score of morphological parameters was significantly higher as a result of 37·5 % SBM inclusion in the diet, while the scores of fish fed 25 % SBM or lower were not different from those of the fish meal-fed controls (P < 0·05). SBMIE was found in the distal intestine (DI) in 18 % of the fish (eleven of sixty): ten in the 37·5 % SBM-fed group and one in the 25 % SBM-fed group. Sugar markers in plasma showed large variation among individuals probably due to variation in feed intake. We found, however, a significant linear increase in the level of plasma d-lactate with increasing SBM inclusion level (P < 0·0001). Plasma concentration of endotoxin was not significantly different in groups with or without SBMIE. Some individual fish showed high values of endotoxin in blood, but the same individuals did not show any bacterial translocation. Plasma bacterial DNA was detected in 28 % of the fish with SBMIE, and 8 % of non-SBMIE fish (P = 0·07). Plasma concentration of d-lactate was significantly higher in fish with SBMIE (P < 0·0001). To conclude, SBMIE in the DI of rainbow trout was associated with an increase in bacterial translocation and plasma d-lactate concentration, suggesting that these permeability markers can be used to evaluate intestinal permeability in vivo.

AB - The primary aim of this experiment was to evaluate the intestinal barrier permeability in vivo in rainbow trout (Oncorhynchus mykiss) fed increasing levels of soyabean meal (SBM). The relationship between SBM-induced enteritis (SBMIE) and the permeability markers was also investigated. Our results showed that the mean score of morphological parameters was significantly higher as a result of 37·5 % SBM inclusion in the diet, while the scores of fish fed 25 % SBM or lower were not different from those of the fish meal-fed controls (P < 0·05). SBMIE was found in the distal intestine (DI) in 18 % of the fish (eleven of sixty): ten in the 37·5 % SBM-fed group and one in the 25 % SBM-fed group. Sugar markers in plasma showed large variation among individuals probably due to variation in feed intake. We found, however, a significant linear increase in the level of plasma d-lactate with increasing SBM inclusion level (P < 0·0001). Plasma concentration of endotoxin was not significantly different in groups with or without SBMIE. Some individual fish showed high values of endotoxin in blood, but the same individuals did not show any bacterial translocation. Plasma bacterial DNA was detected in 28 % of the fish with SBMIE, and 8 % of non-SBMIE fish (P = 0·07). Plasma concentration of d-lactate was significantly higher in fish with SBMIE (P < 0·0001). To conclude, SBMIE in the DI of rainbow trout was associated with an increase in bacterial translocation and plasma d-lactate concentration, suggesting that these permeability markers can be used to evaluate intestinal permeability in vivo.

KW - rainbow trout

KW - soyabean meal

KW - enteritis

KW - Intestinal permeability

KW - permeability markers

U2 - 10.1017/jns.2016.7

DO - 10.1017/jns.2016.7

M3 - Article

VL - 5

JO - Journal of Nutritional Science

JF - Journal of Nutritional Science

SN - 2048-6790

M1 - e26

ER -