Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH

Sidnei C. Santos, Martin G. Liebensteiner, Antonie H. van Gelder, Mauricio R. Dimitrov, Paulo F. Almeida, Cristina M. Quintella, Alfons J.M. Stams, Irene Sánchez-Andrea

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Glycerol is a main co-product of biodiesel production. Crude glycerol may serve as a cheap and attractive substrate in biotechnological applications, e.g. for the production of valuable chemicals or as an electron donor for reduction processes. In this work, sulfate reduction with glycerol was studied at neutral and acidic pH using bioreactor sludge samples and Tinto River sediments as a source of inoculum, respectively. Communities of sulfate-reducing bacteria (SRB) and fermentative bacteria were co-enriched at both pH values. Molecular analyses revealed that sequences belonging to Desulfomicrobium genus were dominant in the cultures enriched at pH 7, while Desulfosporosinus sequences dominated in the culture enriched at pH 4. Glycerol conversion was coupled to sulfate reduction, but the substrate was incompletely oxidized to acetate in the neutrophilic enrichments, and acetate, lactate, and 1,3-propanediol under low pH conditions. Two strains belonging to Desulfomicrobium and Proteiniphilum genera were isolated from the neutrophilic enrichments, but the first isolate was not able to use glycerol, which suggests a syntrophic relationship between glycerol-degrading fermentative bacteria and SRB. A Clostridium strain able to grow with glycerol was isolated from the low pH enrichment. Our data indicate that glycerol promotes the growth of sulfate- reducing communities to form sulfide, which can be used to precipitate and recover heavy metals.
Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalJournal of General and Applied Microbiology
Volume64
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

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Glycerol
Sulfates
Bacteria
Acetates
Biofuels
Clostridium
Bioreactors
Sulfides
Heavy Metals
Sewage
Rivers
Sequence Analysis
Lactic Acid
Electrons
Growth

Keywords

  • Fermentative bacteria
  • Glycerol
  • Metal recovery
  • Sulfate-reducing bacteria
  • Syntrophy

Cite this

Santos, S. C., Liebensteiner, M. G., van Gelder, A. H., Dimitrov, M. R., Almeida, P. F., Quintella, C. M., ... Sánchez-Andrea, I. (2018). Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH. Journal of General and Applied Microbiology, 64(1), 1-8. https://doi.org/10.2323/jgam.2017.02.009
Santos, Sidnei C. ; Liebensteiner, Martin G. ; van Gelder, Antonie H. ; Dimitrov, Mauricio R. ; Almeida, Paulo F. ; Quintella, Cristina M. ; Stams, Alfons J.M. ; Sánchez-Andrea, Irene. / Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH. In: Journal of General and Applied Microbiology. 2018 ; Vol. 64, No. 1. pp. 1-8.
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Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH. / Santos, Sidnei C.; Liebensteiner, Martin G.; van Gelder, Antonie H.; Dimitrov, Mauricio R.; Almeida, Paulo F.; Quintella, Cristina M.; Stams, Alfons J.M.; Sánchez-Andrea, Irene.

In: Journal of General and Applied Microbiology, Vol. 64, No. 1, 01.01.2018, p. 1-8.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH

AU - Santos, Sidnei C.

AU - Liebensteiner, Martin G.

AU - van Gelder, Antonie H.

AU - Dimitrov, Mauricio R.

AU - Almeida, Paulo F.

AU - Quintella, Cristina M.

AU - Stams, Alfons J.M.

AU - Sánchez-Andrea, Irene

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Glycerol is a main co-product of biodiesel production. Crude glycerol may serve as a cheap and attractive substrate in biotechnological applications, e.g. for the production of valuable chemicals or as an electron donor for reduction processes. In this work, sulfate reduction with glycerol was studied at neutral and acidic pH using bioreactor sludge samples and Tinto River sediments as a source of inoculum, respectively. Communities of sulfate-reducing bacteria (SRB) and fermentative bacteria were co-enriched at both pH values. Molecular analyses revealed that sequences belonging to Desulfomicrobium genus were dominant in the cultures enriched at pH 7, while Desulfosporosinus sequences dominated in the culture enriched at pH 4. Glycerol conversion was coupled to sulfate reduction, but the substrate was incompletely oxidized to acetate in the neutrophilic enrichments, and acetate, lactate, and 1,3-propanediol under low pH conditions. Two strains belonging to Desulfomicrobium and Proteiniphilum genera were isolated from the neutrophilic enrichments, but the first isolate was not able to use glycerol, which suggests a syntrophic relationship between glycerol-degrading fermentative bacteria and SRB. A Clostridium strain able to grow with glycerol was isolated from the low pH enrichment. Our data indicate that glycerol promotes the growth of sulfate- reducing communities to form sulfide, which can be used to precipitate and recover heavy metals.

AB - Glycerol is a main co-product of biodiesel production. Crude glycerol may serve as a cheap and attractive substrate in biotechnological applications, e.g. for the production of valuable chemicals or as an electron donor for reduction processes. In this work, sulfate reduction with glycerol was studied at neutral and acidic pH using bioreactor sludge samples and Tinto River sediments as a source of inoculum, respectively. Communities of sulfate-reducing bacteria (SRB) and fermentative bacteria were co-enriched at both pH values. Molecular analyses revealed that sequences belonging to Desulfomicrobium genus were dominant in the cultures enriched at pH 7, while Desulfosporosinus sequences dominated in the culture enriched at pH 4. Glycerol conversion was coupled to sulfate reduction, but the substrate was incompletely oxidized to acetate in the neutrophilic enrichments, and acetate, lactate, and 1,3-propanediol under low pH conditions. Two strains belonging to Desulfomicrobium and Proteiniphilum genera were isolated from the neutrophilic enrichments, but the first isolate was not able to use glycerol, which suggests a syntrophic relationship between glycerol-degrading fermentative bacteria and SRB. A Clostridium strain able to grow with glycerol was isolated from the low pH enrichment. Our data indicate that glycerol promotes the growth of sulfate- reducing communities to form sulfide, which can be used to precipitate and recover heavy metals.

KW - Fermentative bacteria

KW - Glycerol

KW - Metal recovery

KW - Sulfate-reducing bacteria

KW - Syntrophy

U2 - 10.2323/jgam.2017.02.009

DO - 10.2323/jgam.2017.02.009

M3 - Article

VL - 64

SP - 1

EP - 8

JO - Journal of General and Applied Microbiology

JF - Journal of General and Applied Microbiology

SN - 0022-1260

IS - 1

ER -

Santos SC, Liebensteiner MG, van Gelder AH, Dimitrov MR, Almeida PF, Quintella CM et al. Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH. Journal of General and Applied Microbiology. 2018 Jan 1;64(1):1-8. https://doi.org/10.2323/jgam.2017.02.009