Bacterial consortium proteomics under 4-chlorosalicylate carbon-limiting conditions

Roberto A. Bobadilla Fazzini, Agata Bielecka, Ana K. Poucas Quintas, Peter N. Golyshin, Maria J. Preto, Kenneth N. Timmis, Vitor A.P. Martins Dos Santos

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

In this study, the stable consortium composed by Pseudomonas reinekei strain MT1 and Achromohacter xylosoxidans strain MT3 (cell numbers in proportion 9:1) was under investigation to reveal bacterial interactions that take place under severe nutrient-limiting conditions. The analysis of steady states in continuous cultures was carried out at the proteome, metabolic profile, and population dynamic levels. Carbon-limiting studies showed a higher metabolic versatility in the community through upregulation of parallel catabolic enzymes (salicylate 5-hydroxylase and 17-fold on 2-keto-4-pentenoate hydratase) indicating a possible alternative carbon routing in the upper degradation pathway highlighting the effect of minor proportions of strain MT3 over the major consortia component strain MT1 with a significant change in the expression levels of the enzymes of the mainly induced biodegradation pathway such as salicylate 1-hydroxylase and catechol 1,2-dioxygenase together with important changes in the outer membrane composition of P. rtinekti MT1 under different culture conditions. The study has demonstrated the importance of the outer membrane as a sensing/response protective barrier caused by interspecies interactions highlighting the role of the major outer membrane proteins OprF and porin D in P. rtinekti sp. MT1 under the culture conditions tested.

Original languageEnglish
Pages (from-to)2273-2285
Number of pages13
JournalProteomics
Volume9
Issue number8
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Keywords

  • Bacterial community
  • Bacterial interactions
  • Carbon-limiting conditions

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