Bacillus thuringiensis delta-endotoxin Cry1Ac domain III enhances activity against Heliothis virescens in some, but not all Cry1-Cry1Ac hybrids

R.B. Karlova; W.M.J. Weemen; S. Naimov; J. Ceron; S. Dukiandjiev; R.A. de Maagd

doi:10.1016/j.jip.2004.11.004

Bacillus thuringiensis delta-endotoxin Cry1Ac domain III enhances activity against Heliothis virescens in some, but not all Cry1-Cry1Ac hybrids

R.B. Karlova, W.M.J. Weemen, S. Naimov, J. Ceron, S. Dukiandjiev, R.A. de Maagd

Biochemistry

Research output: Contribution to journal › Article › Academic › peer-review

28 Citations (Scopus)

Abstract

We investigated the role of domain III of Bacillus thuringiensis d-endotoxin Cry1Ac in determining toxicity against Heliothis virescens. Hybrid toxins, containing domain III of Cry1Ac with domains I and II of Cry1Ba, Cry1Ca, Cry1Da, Cry1Ea, and Cry1Fb, respectively, were created. In this way Cry1Ca, Cry1Fb, and to a lesser extent Cry1Ba were made considerably more toxic.

Original language	English
Pages (from-to)	169-172
Journal	Journal of Invertebrate Pathology
Volume	88
Issue number	2
DOIs	https://doi.org/10.1016/j.jip.2004.11.004
Publication status	Published - 2005

Keywords

aminopeptidase-n-receptor
spodoptera-exigua
crystal proteins
insect midgut
specificity
binding
toxin
membrane
larvae
acetylgalactosamine

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10.1016/j.jip.2004.11.004

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title = "Bacillus thuringiensis delta-endotoxin Cry1Ac domain III enhances activity against Heliothis virescens in some, but not all Cry1-Cry1Ac hybrids",

abstract = "We investigated the role of domain III of Bacillus thuringiensis d-endotoxin Cry1Ac in determining toxicity against Heliothis virescens. Hybrid toxins, containing domain III of Cry1Ac with domains I and II of Cry1Ba, Cry1Ca, Cry1Da, Cry1Ea, and Cry1Fb, respectively, were created. In this way Cry1Ca, Cry1Fb, and to a lesser extent Cry1Ba were made considerably more toxic.",

keywords = "aminopeptidase-n-receptor, spodoptera-exigua, crystal proteins, insect midgut, specificity, binding, toxin, membrane, larvae, acetylgalactosamine",

author = "R.B. Karlova and W.M.J. Weemen and S. Naimov and J. Ceron and S. Dukiandjiev and {de Maagd}, R.A.",

year = "2005",

doi = "10.1016/j.jip.2004.11.004",

language = "English",

volume = "88",

pages = "169--172",

journal = "Journal of Invertebrate Pathology",

issn = "0022-2011",

publisher = "Elsevier",

number = "2",

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TY - JOUR

T1 - Bacillus thuringiensis delta-endotoxin Cry1Ac domain III enhances activity against Heliothis virescens in some, but not all Cry1-Cry1Ac hybrids

AU - Karlova, R.B.

AU - Weemen, W.M.J.

AU - Naimov, S.

AU - Ceron, J.

AU - Dukiandjiev, S.

AU - de Maagd, R.A.

PY - 2005

Y1 - 2005

N2 - We investigated the role of domain III of Bacillus thuringiensis d-endotoxin Cry1Ac in determining toxicity against Heliothis virescens. Hybrid toxins, containing domain III of Cry1Ac with domains I and II of Cry1Ba, Cry1Ca, Cry1Da, Cry1Ea, and Cry1Fb, respectively, were created. In this way Cry1Ca, Cry1Fb, and to a lesser extent Cry1Ba were made considerably more toxic.

AB - We investigated the role of domain III of Bacillus thuringiensis d-endotoxin Cry1Ac in determining toxicity against Heliothis virescens. Hybrid toxins, containing domain III of Cry1Ac with domains I and II of Cry1Ba, Cry1Ca, Cry1Da, Cry1Ea, and Cry1Fb, respectively, were created. In this way Cry1Ca, Cry1Fb, and to a lesser extent Cry1Ba were made considerably more toxic.

KW - aminopeptidase-n-receptor

KW - spodoptera-exigua

KW - crystal proteins

KW - insect midgut

KW - specificity

KW - binding

KW - toxin

KW - membrane

KW - larvae

KW - acetylgalactosamine

U2 - 10.1016/j.jip.2004.11.004

DO - 10.1016/j.jip.2004.11.004

M3 - Article

SN - 0022-2011

VL - 88

SP - 169

EP - 172

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

IS - 2

ER -

Bacillus thuringiensis delta-endotoxin Cry1Ac domain III enhances activity against Heliothis virescens in some, but not all Cry1-Cry1Ac hybrids

Abstract

Keywords

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